Summary of Study ST000309

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000248. The data can be accessed directly via it's Project DOI: 10.21228/M8QS3G This work is supported by NIH grant, U2C- DK119886.


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Study IDST000309
Study TitleMetabolome of three Vancomycin-intermediate Staphylococcus aureus (VISA) mutants compared with the parent strain MM66
Study SummaryVancomycin-intermediate Staphylococcus aureus (VISA) evolve in a strain-specific manner and acquire mutations that lead to alterations in cell wall metabolism that reduce susceptibility to vancomycin. We had earlier isolated several VISA mutant strains of the clinical hVISA strain MM66. This study is aimed at analyzing the metabolome of these mutants in comparison to the parent strain.
Oklahoma State University
Last NameGustafson
First NameJohn
Address246C Noble Research Center Oklahoma State University Stillwater, OK 74078-3035
Submit Date2016-01-12
Raw Data File Type(s)d
Analysis Type DetailGC-MS
Release Date2016-01-14
Release Version1
John Gustafson John Gustafson application/zip

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Sample Preparation:

Sampleprep ID:SP000337
Sampleprep Summary:The sample extraction was referred to the procedure of Roessner et al. (2000, 2001) with minor changes: About 10 mg (DW) of S. aureus cells were crashed in the mill (Model, Manufacturer), and then sonicated with the addition of 1.5 ml of 70% methanol for 10 min at 600C, followed by extraction with 1.5 ml water at room temperature. Both extracts were combined and dried in vacuum. Prior to GC/MS analysis, dried polar extracts were derivatized with 80 µl Methoxyamine hydrochloride (20mg ml-1) for 60 min at 400C and then with 80 µl MSTFA at 650C for 40 min.