Summary of Study ST000408

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000320. The data can be accessed directly via it's Project DOI: 10.21228/M8MP4W This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis | Show all samples | Download binned data
Download mwTab file (text) | Download mwTab file(JSON) | Download data files (Contains raw data)

Study ID	ST000408
Study Title	Metabolomic analysis of oxytocin effects on social deficits in mice (part I)
Study Summary	The goal of this study is to determine the prosocial hormone oxytocin (OT) effects on metabolomic profiles in brain.
Institute	University of North Carolina
Laboratory	Sumner Lab
Last Name	Sumner
First Name	Susan
Address	Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Email	susan_sumner @unc.edu
Phone	704-250-5066
Submit Date	2016-06-02
Raw Data Available	Yes
Raw Data File Type(s)	1r
Analysis Type Detail	NMR
Release Date	2018-06-05
Release Version	1

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:	SP000436
Sampleprep Summary:	Each thawed tissue sample was transferred into a pre-labeled MagnaLyser tube and massed. 50% acetonitrile in water was added depending on the mass of each sample (500 uL for up to 200 mg and 1000 uL for more than 200 mg). Samples were homogenized using MagnaLyser for two 30 sec pulses and then centrifuged. A volume of each sample supernatant required to analyze the desired sample mass (20 mg for Mid, Cerb, Hind, Fore, and 10 mg for Olf) was aliquoted. For a subset of samples with excess material, a total study pool was created by mixing the remaining volume of extracted tissue. Five replicates of the total study pool were created and prepared identically to the study samples. Samples were frozen and lyophilized. Lyophilized samples were mixed with 250 μl of Master mix (0.2 mM phosphate buffer (pH 7.5) containing 10% Chenomx Internal Standard - 0.5 mM DSS-d6 and 6mM Imidazole). The tubes were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 4 min. A 200 µl aliquot of the supernatant was transferred into pre-labeled 3 mm (4”) NMR tubes for data acquisition on a 700 MHz spectrometer.

Sampleprep ID:

SP000436

Sampleprep Summary:

Each thawed tissue sample was transferred into a pre-labeled MagnaLyser tube and massed. 50% acetonitrile in water was added depending on the mass of each sample (500 uL for up to 200 mg and 1000 uL for more than 200 mg). Samples were homogenized using MagnaLyser for two 30 sec pulses and then centrifuged. A volume of each sample supernatant required to analyze the desired sample mass (20 mg for Mid, Cerb, Hind, Fore, and 10 mg for Olf) was aliquoted. For a subset of samples with excess material, a total study pool was created by mixing the remaining volume of extracted tissue. Five replicates of the total study pool were created and prepared identically to the study samples. Samples were frozen and lyophilized. Lyophilized samples were mixed with 250 μl of Master mix (0.2 mM phosphate buffer (pH 7.5) containing 10% Chenomx Internal Standard - 0.5 mM DSS-d6 and 6mM Imidazole). The tubes were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 4 min. A 200 µl aliquot of the supernatant was transferred into pre-labeled 3 mm (4”) NMR tubes for data acquisition on a 700 MHz spectrometer.