Summary of Study ST000410

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000320. The data can be accessed directly via it's Project DOI: 10.21228/M8MP4W This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST000410
Study Title	Metabolomic analysis of oxytocin effects on social deficits in mice (part III)
Study Summary	The goal of this study is to determine the prosocial hormone oxytocin (OT) effects on metabolomic profiles in brain.
Institute	University of North Carolina
Laboratory	Sumner Lab
Last Name	Sumner
First Name	Susan
Address	Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Email	susan_sumner @unc.edu
Phone	704-250-5066
Submit Date	2016-06-17
Raw Data Available	Yes
Raw Data File Type(s)	cdf
Analysis Type Detail	NMR
Release Date	2017-07-10
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP000438
Sampleprep Summary:	The NMR samples were used for the neurotransmitter analysis after NMR data acquisition. These samples were lyophilized overnight followed by reconstitution in 50 µL 95:5 methanol:water. Following a final centrifugation, extracts were transferred to vials for analysis by injection (20 µL) onto the Thermo Scientific (TS) 3000 series U-HPLC system. Compound separation was achieved on a Thermo Scientific Hypersil Gold, 200 x 2.1 mm column, and 1.9 µm particle size with an isocratic flow rate of 0.400 mL/min with a column temperature of 30°C. Simultaneous detection of neurotransmitters was achieved by the TS 5600A 16 channel coulometric array ECD with coulometric cells set at incremental potentials ranging from -150 mV to 900 mV. A Standard Mix containing 14 target compounds at known concentrations was analyzed just prior to each batch to confirm the retention time and elution channel of each compound. Additionally, the mix was used as an external standard to semi-quantitate any targets found in the extracts.

Sampleprep ID:

SP000438

Sampleprep Summary:

The NMR samples were used for the neurotransmitter analysis after NMR data acquisition. These samples were lyophilized overnight followed by reconstitution in 50 µL 95:5 methanol:water. Following a final centrifugation, extracts were transferred to vials for analysis by injection (20 µL) onto the Thermo Scientific (TS) 3000 series U-HPLC system. Compound separation was achieved on a Thermo Scientific Hypersil Gold, 200 x 2.1 mm column, and 1.9 µm particle size with an isocratic flow rate of 0.400 mL/min with a column temperature of 30°C. Simultaneous detection of neurotransmitters was achieved by the TS 5600A 16 channel coulometric array ECD with coulometric cells set at incremental potentials ranging from -150 mV to 900 mV. A Standard Mix containing 14 target compounds at known concentrations was analyzed just prior to each batch to confirm the retention time and elution channel of each compound. Additionally, the mix was used as an external standard to semi-quantitate any targets found in the extracts.