Summary of Study ST000481
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000353. The data can be accessed directly via it's Project DOI: 10.21228/M8DW3Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000481 |
| Study Title | Metabolomics of Mice Cohousing and Microbiota Transfer (part II) |
| Study Summary | The mice serum samples were extracted and analyzed using broad spectrum GCMS for the identification of compounds distinguishing the groups. |
| Institute | University of North Carolina |
| Department | Discovery-Sciences-Technology (DST) |
| Laboratory | Sumner Lab |
| Last Name | Sumner |
| First Name | Susan |
| Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
| susan_sumner @unc.edu | |
| Phone | 704-250-5066 |
| Submit Date | 2016-08-25 |
| Num Groups | 4 |
| Total Subjects | 12 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | GC-MS |
| Release Date | 2018-04-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP000931 |
| Sampleprep Summary: | Extraction, Solvent Removal, Resuspension, Solvent Removal, Two-step Derivatization and Addition of FAME Markers |
| Processing Method: | 30 µL of serum were added to Eppendorf tube. Add 1000 µL of 3:3:2 ACN:ISP:H20 extraction solution to aliquots, shake and centrifuge for 2 minutes at 4C and 14000 rcf to precipitate proteins. Sample was split into (2)-450 µL fractions, one fraction was archived in -80°C the second fraction was subject t complete sample processing. Transfer supernatant to a new vial. Evaporate supernatant to complete dryness at room temp. |
| Processing Storage Conditions: | On Ice and or 4⁰C cold room |
| Extraction Method: | (step 1) 3:3:2 Acetonitrile:Isopropanol:H2O, (step 2) 1:1 Acetonitrile:H2O |
| Extract Storage: | -80⁰C |
| Sample Resuspension: | Samples resuspended in 200 µL of 1:1 ACN:H2O. Evaporated to dryness w/speed vac. |
| Sample Derivatization: | Formation of methoximes by adding 10µL of 40mg/mL MeOx in pyridine to each sample. Placed onto Thermomixer for 90 min at 30⁰C. Then FAME retention index markers and MSTFA added to each sample for derivatization step. Samples returned to Thermomixer for 45 min at 70⁰C. |
| Sample Spiking: | Fatty acid methyl esters (FAME) |
