Summary of Study ST000487

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000369. The data can be accessed directly via it's Project DOI: 10.21228/M8BW2Q This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST000487
Study Title	Impacts of high-fat diet (HFD), high-carbohydrate diet (HCD) and high-fat-high-carbohydrate diet (HFHCD) on metabolites in a farmed cyprinid fish Megalobrama amblycephala.
Study Type	Dietary imbalance experiment
Study Summary	1H NMR-based metabolomic approach to measure the concentrations of metabolites in plasma and liver of four different diet groups: HFD, HCD, HFHCD and control. Multivariate statistical analyses were used to determine significantly changed metabolites between all group-pairs.
Institute	Huazhong Agricultural University
Department	Fisheries
Laboratory	Key Lab of Freshwater Animal Breeding
Last Name	Prathomya
First Name	Panita
Address	Huazhong Agricultural University, Shizi Shan Jie, Hongshan District.
Email	p_prathomya@yahoo.com
Phone	+8613477033229
Submit Date	2016-10-08
Raw Data File Type(s)	fid
Analysis Type Detail	NMR
Release Date	2017-10-03
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP000515
Sampleprep Summary:	Each plasma sample (170 μL) was mixed with 340 μL phosphate buffer (45 mM, pH 7.47, 50% D2O) containing 0.9% NaCl in a 5 mm NMR tube and used directly for the NMR analysis. Liver tissues (about 50 mg) were homogenized in cold methanol and water (v/v=2:1) using a Qiagen Tissue-Lyser (Retsch GmBH, Germany). The supernatant (550uL) was collected after centrifugation (16 099 g, 4°C, 10 min). The extraction was repeated thrice using the same procedure. The supernatants collected from the above procedure were mixed together and freeze-dried after the removal of methanol in vacuo. Then the residue was dissolved in 600 μL of phosphate buffer (0.15 M, pH 7.38, 80% D2O) containing 0.001% TSP and 0.01% NaN3. The supernatant (550 μL) was transferred into a 5 mm NMR tube.

Sampleprep ID:

SP000515

Sampleprep Summary:

Each plasma sample (170 μL) was mixed with 340 μL phosphate buffer (45 mM, pH 7.47, 50% D2O) containing 0.9% NaCl in a 5 mm NMR tube and used directly for the NMR analysis. Liver tissues (about 50 mg) were homogenized in cold methanol and water (v/v=2:1) using a Qiagen Tissue-Lyser (Retsch GmBH, Germany). The supernatant (550uL) was collected after centrifugation (16 099 g, 4°C, 10 min). The extraction was repeated thrice using the same procedure. The supernatants collected from the above procedure were mixed together and freeze-dried after the removal of methanol in vacuo. Then the residue was dissolved in 600 μL of phosphate buffer (0.15 M, pH 7.38, 80% D2O) containing 0.001% TSP and 0.01% NaN3. The supernatant (550 μL) was transferred into a 5 mm NMR tube.