Summary of Study ST000546
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000400. The data can be accessed directly via it's Project DOI: 10.21228/M8BS4F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000546 |
| Study Title | Multi-omics based identification of specific biochemical changes associated with PfKelch13-mutant artemisinin resistant Plasmodium |
| Study Type | Cell type comparison |
| Study Summary | Two clonaly artemisinin resistant parasitised red blood cells (trophozoite stage) were compared with artemsinin sensitive parasitised red blood cells by metabolomics analysis. |
| Institute | Monash University |
| Department | Monash Institute of Pharmaceutical Sciences, Drug Delivery, Disposition and Dynamics |
| Laboratory | Creek lab |
| Last Name | Creek |
| First Name | Darren |
| Address | 381 Royal Parade, Parkville, Melbourne, VIC3052, Australia |
| Darren.Creek@monash.edu | |
| Phone | N/A |
| Submit Date | 2017-01-23 |
| Num Groups | 4 |
| Total Subjects | 13 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-07-10 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP000575 |
| Sampleprep Summary: | Metabolism was quenched by washing with ice cold PBS (1 mL). Cells were then pelleted by centrifugation (6000 xg for 3 min) and metabolites were extracted from 5 x 107 uninfected red blood cells and 5 x 107 parasitised red blood cells reticulocytes by addition of 140 µL methanol containing internal standards (CHAPS, CAPS, PIPES and TRIS; 1 µM) and left for 1 hour at 4 °C with automatic vortex. After mixing, cellular debris was removed by centrifugation (16,000 rpm for 10 mins) and the supernatant was kept at -20 °C prior to analysis. |
| Processing Method: | Lysis with automatic vortex at 4°C |
| Processing Storage Conditions: | On ice or 4°C |
| Extraction Method: | Methanol |
| Extract Storage: | -20°C |
| Sample Spiking: | Internal standards (CHAPS, CAPS, PIPES and TRIS; all at 1 µM) mixed in extraction solvent |
| Cell Type: | Artemisinin resistant and sensitive parasitised red blood cells and uninfected red blood cells |
