Summary of Study ST000603
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000440. The data can be accessed directly via it's Project DOI: 10.21228/M85W25 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000603 |
Study Title | Urinary Volatile Compound, Associated with Chronic Inflammation In Interstitial Cystitis |
Study Summary | Interstitial cystitis (IC)/bladder pain syndrome (BPS) is a clinical condition that manifests as a sensory hypersensitivity of unknown cause and is characterized by urinary frequency, bladder discomfort, and pelvic pain. In the present volatolomic study, we have analyzed the VOCs unique to urine specimens obtained from interstitial cystitis patients, in compassion to healthy controls.This is the novel finding from comprehensive and unbiased metabolomics analysis that urinary menthol is decreased in urine specimens from IC patients, and that the reduced menthol level in IC is potentially linked to the chronic inflammation, which is often observed in IC patients |
Institute | University of California, Davis |
Department | Genome and Biomedical Sciences Facility |
Laboratory | WCMC Metabolomics Core |
Last Name | Fiehn |
First Name | Oliver |
Address | Health Sciences Drive, Davis, California, 95616, USA |
ofiehn@ucdavis.edu | |
Phone | (530) 754-8258 |
Submit Date | 2017-05-09 |
Raw Data Available | Yes |
Raw Data File Type(s) | cdf |
Analysis Type Detail | GC-MS |
Release Date | 2017-07-10 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP000633 |
Sampleprep Summary: | 1. Vortex the urine samples for about 10s to obtain a homogenized sample using the MiniVortexer VWR. 2. Aliquot 15-30ul and add 1ml extraction solution. The extraction solution has to be pre-chilled using the ThermoElectron Neslab RTE 740 cooling bath set to -20°C. 3. Vortex the sample for about 10s and shake for 5min at 4°C using the Orbital Mixing Chilling/Heating plate. If you are using more than one sample, keep the rest of the samples on ice (chilled at <0°C with NaCl). 4. Centrifuge samples for 2min at 14000 rcf using the centrifuge Eppendorf 5415 D. 5. Aliquot 2X 0.5ml supernatant. One for analysis and one for backup. Store 2nd aliquot in -20°C freezer. 6. Evaporate 0.5ml aliquots of the sample in the Labconco Centrivap cold trap concentrator to complete dryness. 7. Submit to derivatization. |
Sampleprep Protocol Filename: | SOP_Extraction_of_Urine.pdf |