Summary of Study ST000815

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000580. The data can be accessed directly via it's Project DOI: 10.21228/M8PX0D This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST000815
Study Title	db/db WT ozone and air exposed mice
Study Summary	The goal of this study was to characterize the metabolic impact manifested in mouse lung following acute ozone exposure, comparing differential effects experienced by lean and obese model mice.
Institute	Harvard School of Public Health
Last Name	Mathews
First Name	Joel
Address	1 DNA Way
Email	joel.mathews@gmail.com
Phone	3019283058
Submit Date	2017-06-05
Analysis Type Detail	GC-MS/LC-MS
Release Date	2017-10-11
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP000848
Sampleprep Summary:	The automated MicroLab STAR® system from Hamilton Company was used to prepare samples for metabolomics profiling. Equal weight of lungs was used from each mouse. Prior to the first step in the extraction process, recovery standards were added for quality control (QC) purposes. A series of organic and aqueous extractions proprietary to Metabolon Inc. was then used to remove protein while allowing maximum recovery of small molecules. Extracts were divided into two fractions. One was used for analysis by liquid chromatography (LC) and the other was used for analysis by gas chromatography (GC). Samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent, frozen, and dried under vacuum. Samples were then prepared for the appropriate instrument, either LC/MS (mass spectrometry) or GC/MS, as described by Evans et al {Evans, 2009 #5074}. For a brief description of the methods used for LC/MS and GC/MS, see the online supplement. After analysis, raw data were extracted, peak-identified and QC processed using Metabolon's proprietary hardware and software. At the time of this analysis, identification of known chemical entities was based on comparison to metabolomic libraries of more than 1000 commercially available purified standard compounds. The combination of chromatographic properties and mass spectra gave an indication of a match to the specific compound or an isobaric entity.

Sampleprep ID:

SP000848

Sampleprep Summary:

The automated MicroLab STAR® system from Hamilton Company was used to prepare samples for metabolomics profiling. Equal weight of lungs was used from each mouse. Prior to the first step in the extraction process, recovery standards were added for quality control (QC) purposes. A series of organic and aqueous extractions proprietary to Metabolon Inc. was then used to remove protein while allowing maximum recovery of small molecules. Extracts were divided into two fractions. One was used for analysis by liquid chromatography (LC) and the other was used for analysis by gas chromatography (GC). Samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent, frozen, and dried under vacuum. Samples were then prepared for the appropriate instrument, either LC/MS (mass spectrometry) or GC/MS, as described by Evans et al {Evans, 2009 #5074}. For a brief description of the methods used for LC/MS and GC/MS, see the online supplement. After analysis, raw data were extracted, peak-identified and QC processed using Metabolon's proprietary hardware and software. At the time of this analysis, identification of known chemical entities was based on comparison to metabolomic libraries of more than 1000 commercially available purified standard compounds. The combination of chromatographic properties and mass spectra gave an indication of a match to the specific compound or an isobaric entity.