Summary of Study ST000886
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000615. The data can be accessed directly via it's Project DOI: 10.21228/M85Q46 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000886 |
| Study Title | Mechanism Behind Stay Green Trait in Bread Wheat (Triticum aestivum L.) |
| Study Type | Comparison |
| Study Summary | Two different wheat genotypes were treated with the high temperature and control conditions under full irrigated condition. Leaf tissues were collected for all 2-different treatments with six replicates after 7 and 10 days of high temperature treatment. |
| Institute | University of Florida |
| Department | Agronomy |
| Last Name | Babar |
| First Name | Ali |
| Address | 3105 Mccarty Hall, gainesville, FL 32611 |
| mababar@ufl.edu | |
| Phone | 217-552-2346 |
| Submit Date | 2017-09-21 |
| Num Groups | 8 |
| Total Subjects | 48 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-10-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP000927 |
| Sampleprep Summary: | Weigh 30mg of the lyophilized and pulverized sample into clean Eppendorf tube and record weight. Add 20μL of Metabolites Internal Standard Mix to each sample. Add 750 μL of Methanol and 750 μL of 10mM Ammonium Acetate to each sample. Homogenize for 30 seconds at 1800 rpm on the BeadBeater. Sonicate for 10-20 minutes at room temperature. Centrifuge at room temperature for 10 minutes at 17,000 xg. Transfer 50 μL of supernatant to a labeled LC vial with a fused insert. Transfer the rest of the supernatant to a labeled 2 mL LC Vial with no fused insert to store in -80°C. |
| Sampleprep Protocol Filename: | Plant_Tissue_SamplePrep_Instrument_method.pdf Appendix_A -_Internal_Standard_Prep_GLCMS.pdf |
