Summary of Study ST000886

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000615. The data can be accessed directly via it's Project DOI: 10.21228/M85Q46 This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000886
Study TitleMechanism Behind Stay Green Trait in Bread Wheat (Triticum aestivum L.)
Study TypeComparison
Study SummaryTwo different wheat genotypes were treated with the high temperature and control conditions under full irrigated condition. Leaf tissues were collected for all 2-different treatments with six replicates after 7 and 10 days of high temperature treatment.
University of Florida
Last NameBabar
First NameAli
Address3105 Mccarty Hall, gainesville, FL 32611
Submit Date2017-09-21
Num Groups8
Total Subjects48
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2018-10-10
Release Version1
Ali Babar Ali Babar application/zip

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Sample Preparation:

Sampleprep ID:SP000927
Sampleprep Summary:Weigh 30mg of the lyophilized and pulverized sample into clean Eppendorf tube and record weight. Add 20μL of Metabolites Internal Standard Mix to each sample. Add 750 μL of Methanol and 750 μL of 10mM Ammonium Acetate to each sample. Homogenize for 30 seconds at 1800 rpm on the BeadBeater. Sonicate for 10-20 minutes at room temperature. Centrifuge at room temperature for 10 minutes at 17,000 xg. Transfer 50 μL of supernatant to a labeled LC vial with a fused insert. Transfer the rest of the supernatant to a labeled 2 mL LC Vial with no fused insert to store in -80°C.
Sampleprep Protocol Filename:Plant_Tissue_SamplePrep_Instrument_method.pdf
Appendix_A -_Internal_Standard_Prep_GLCMS.pdf