Summary of Study ST001101

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000737. The data can be accessed directly via it's Project DOI: 10.21228/M8DM5Q This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST001101
Study Title	Identification of potential sepsis biomarkers in burn injury conditions.
Study Summary	12 mice were subjected to one of four treatment groups: no burn injury:no injection, burn injury:no infection, no burn injury:infection, and burn injury:infection. The researchers in this study are looking for metabolites in serum that could indicate sepsis in mice with burn injuries.
Institute	Texas Tech University
Department	Department of Biological Sciences
Last Name	Elmassry
First Name	Moamen
Address	2901 Main St, Lubbock, TX 79409
Email	moamen.elmassry@ttu.edu
Phone	806-742-2715
Submit Date	2018-11-29
Num Groups	4
Total Subjects	12
Raw Data Available	Yes
Analysis Type Detail	GC-MS
Release Date	2019-01-22
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP001152
Sampleprep Summary:	30µL of plasma was aliquoted into a 1.5 mL eppendorph tube. 1 mL of 3:3:2 ACN:H20:IPA was added to each sample. Samples were vortexed for 10 seconds and then shaken at 4°C for 5 minutes using an orbital mixing plate. Samples were centrifuged for 2 minutes at 14,000 rcf. 2 x 475 µL aliquots were removed, one dried down and submitted to derivitization, the other frozen at -20°C for backup. Samples were derivatized by adding 10 µL of MeOX and then shaken at 30°C for 1.5 hours using a orbital mixing plate. 91 µL of MSTFA + FAMES mixutre is added to each sample. Samples were shaken at 37°C for 30 minutes. Samples submitted to GC analysis.

Sampleprep ID:

SP001152

Sampleprep Summary:

30µL of plasma was aliquoted into a 1.5 mL eppendorph tube. 1 mL of 3:3:2 ACN:H20:IPA was added to each sample. Samples were vortexed for 10 seconds and then shaken at 4°C for 5 minutes using an orbital mixing plate. Samples were centrifuged for 2 minutes at 14,000 rcf. 2 x 475 µL aliquots were removed, one dried down and submitted to derivitization, the other frozen at -20°C for backup. Samples were derivatized by adding 10 µL of MeOX and then shaken at 30°C for 1.5 hours using a orbital mixing plate. 91 µL of MSTFA + FAMES mixutre is added to each sample. Samples were shaken at 37°C for 30 minutes. Samples submitted to GC analysis.