Summary of Study ST001165

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000727. The data can be accessed directly via it's Project DOI: 10.21228/M8Q38G This work is supported by NIH grant, U2C- DK119886.


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Study IDST001165
Study TitlePhysiological and metabolic response of crab megalopae and juveniles to ocean acidification (part-III)
Study SummaryYoung crab samples were placed into 1 of 4 treatment groups to understand their metabolic response to ocean acidification and dissolved oxygen content.
DepartmentCB Division
Last NameNichols
First NameKrista
Submit Date2019-04-05
Analysis Type DetailLC-MS
Release Date2019-05-15
Release Version1
Krista Nichols Krista Nichols application/zip

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Sample Preparation:

Sampleprep ID:SP001238
Sampleprep Summary:15mg of whole crab was placed into a 1.5mL ependorph tube. 2 x 3mm grinding beads were added to each sample. 225 µL of cold MeOH with quality controls was added to each samples. Batches of samples were ground with GenoGrinder for 30 seconds at 1500 rpm. 750µL of methyl tert-butyl Ether (MTBE) was added to each sample. Samples were vortexed for 10 seconds and then shaken at 4°C for 5 minutes using an Orbital Mixer. 188 uL of LC-MS grade water was added to each sample. Vortex for 10 seconds and then centrifuged for 2 minutes at 14,000 rcf. 2 x 350µL aliquots were removed from the top, organic layer, one submitted for analysis and the other stored as backup in -20°C. 2 x 125µL aliquots were removed from the bottom, polar layer, one submitted for analysis, the other stored at -20°C for backup.
Sample Resuspension:After drying, samples were resuspended in 110 µL of 9:1 methanol:toulene with 50 nM CUDA as an internal standard. Samples were vortexed for 10 seconds, then sonicated in room temperature water for 5 minutes, then centrifuged at 16,100 rcf for 2 minutes. 50 µL of the supernatant was removed and placed in an amber vial for lipidomics analysis.