Summary of Study ST001232

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000825. The data can be accessed directly via it's Project DOI: 10.21228/M81X1W This work is supported by NIH grant, U2C- DK119886.

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Study IDST001232
Study TitleCombining stage - specificity and metabolomic profiling to advance drug discovery for malaria
Study TypeDrug Exposure
Study SummaryMetabolic examination/confirmation of MoA stage-specificity observed in study
Institute
Pennsylvania State University
DepartmentBMB
LaboratoryLlinás Laboratory
Last NameOwen
First NameEdward
Address6014 Goldenrod Court
Emaileso5005@psu.edu
Phone8144045235
Submit Date2019-07-09
Analysis Type DetailLC-MS
Release Date2020-03-03
Release Version1
Edward Owen Edward Owen
https://dx.doi.org/10.21228/M81X1W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP001308
Sampleprep Summary:Samples washed in PBS, spun down, aspirated, extracted in 90% methanol with labelled aspartate, then dried, resuspended in water labelled with chlorpropamide
Sampleprep Protocol Filename:Metabolite_Extraction_for_LCMS_2017_Llinas.pdf
Processing Method:Wash, Lysis, Drying, resuspension
Processing Storage Conditions:On ice
Extraction Method:90% Methanol Lysis
Extract Cleanup:Centrifugation, pellet removal
Extract Storage:-80℃
Sample Resuspension:100 uL water with chlorpropamide
Sample Spiking:Labelled Aspartate, Chlorpropamide
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