Summary of Study ST001257
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000843. The data can be accessed directly via it's Project DOI: 10.21228/M8QH5G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001257 |
| Study Title | Nutrimetabolomics and DASH diet |
| Study Summary | Although health benefits of the Dietary Approaches to Stop Hypertension (DASH) diet are established, it is not understood which food compounds result in these benefits. We used a step-wise approach to identify unique compounds from individual foods of a DASH-style diet, determined if these Food-Specific Compounds (FSC) are detectable in urine, and then examined relationships between urinary FSC and blood pressure (BP). Nineteen subjects were randomized into 6-week controlled DASH-style diet interventions. Untargeted, LC/MS-based metabolomics was performed on 24-hour urine samples collected before and after each intervention and on 12 representative DASH-style foods. |
| Institute | University of Colorado Denver |
| Department | Anschutz Medical Campus |
| Last Name | Reisdorph |
| First Name | Nichole |
| Address | 12850 East Montview Blvd, Aurora, CO, 80045, USA |
| nichole.reisdorph@ucdenver.edu | |
| Phone | 3037249234 |
| Submit Date | 2019-09-22 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-09-22 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP001333 |
| Sampleprep Summary: | Individual food and urine samples were stored at -80 °C prior to sample preparation. Individual foods (including apple juice) were individually dried using a FreeZone 2.5 plus lyophilizer (Labconco, Kansas City, MO, USA). Dried samples were then divided and macerated using a chilled bio-pulverizer (BioSpec Products, Bartlesville, OK). Approximately 50 mg of each sample was measured into chilled microcentrifuge tubes and stored at -80o C prior to further processes and analysis. 10 µL of coffee was diluted with 90 µL of water, vortexed, aliquotted and stored at -80° C until analysis. An aliquot of coffee was also reserved for neat (i.e. unprocessed) analysis, and stored at -80° C until analysis. Freeze-dried foods were removed from -80° C and allowed to thaw on ice. To each sample, 480 μl of chilled methanol, 10 µl each of a hydrophilic spike mix, hydrophilic positive control mix, hydrophobic spike mix and hydrophobic positive control mix were added (see Supplemental Table S1). Samples were gently vortexed for 10 seconds, placed at -80o C for 60 minutes, and then centrifuged 0o C at 18,000 x g for 15 minutes to facilitate protein precipitation. Supernatants were transferred to new microcentrifuge tubes and dried using vacuum centrifugation at 45o C for approximately 60 minutes. Each sample was suspended in 50 µl of 95:5 LC/MS grade water-acetonitrile and gently vortexed for 30 seconds. Following a quick centrifugation, each sample was then divided into replicate 20 µl aliquots, while 10 µl was removed from each sample to generate a pooled QC sample. Samples were stored at -80° C until analysis. Urine samples were analyzed neat (i.e. without any preparation) except that authentic standards were spiked into urine samples to monitor variability in instrument response or batch effects. |
| Processing Storage Conditions: | Described in summary |
