Summary of Study ST001290

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000872. The data can be accessed directly via it's Project DOI: 10.21228/M8ZT30 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST001290
Study Title	Lipidome Signatures of Metastasis in a Transgenic Mouse Model of Sonic Hedgehog Medulloblastoma.
Study Type	Untargeted metabolomics
Study Summary	Metabolic alternations were investigated by applying Ultra Performance Liquid Chromatography Mass Spectrometry (UPLC-MS) to mice brain tissue samples collected from SmoA1-Math-GFP mice with (n=18) and without (n=7) metastasis. All samples were analyzed using reverse phase (RP) UPLC-MS analysis in positive and negative ion modes.
Institute	Georgia Institute of Technology
Department	Chemistry
Laboratory	Fernández
Last Name	Huang
First Name	Danning
Address	901 Atlantic Dr NE
Email	dhuang74@gatech.edu
Phone	4045127523
Submit Date	2019-12-13
Num Groups	2
Total Subjects	25
Num Males	5
Num Females	20
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2020-12-16
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP001370
Sampleprep Summary:	Frozen mice brain tissue samples were cryo-pulverized with liquid nitrogen and weighed. 2-propanol (20 uL/mg) was added to the cryo-pulverized tissue samples to precipitate proteins. Samples were vortex-mixed for 1 min, and centrifuged at 13,000 rpm for 7 min. Following centrifugation, 100 uL of supernatant was transferred to an autosampler vial and analyzed with UPLC-MS. A blank sample, prepared with 100 uL 2-propanol, underwent the same process as the murine tumor samples. A pooled quality control (QC) sample was prepared by mixing a 10 uL aliquot of supernatant of each sample.

Sampleprep ID:

SP001370

Sampleprep Summary:

Frozen mice brain tissue samples were cryo-pulverized with liquid nitrogen and weighed. 2-propanol (20 uL/mg) was added to the cryo-pulverized tissue samples to precipitate proteins. Samples were vortex-mixed for 1 min, and centrifuged at 13,000 rpm for 7 min. Following centrifugation, 100 uL of supernatant was transferred to an autosampler vial and analyzed with UPLC-MS. A blank sample, prepared with 100 uL 2-propanol, underwent the same process as the murine tumor samples. A pooled quality control (QC) sample was prepared by mixing a 10 uL aliquot of supernatant of each sample.