Summary of Study ST001294

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000876. The data can be accessed directly via it's Project DOI: 10.21228/M8FX1M This work is supported by NIH grant, U2C- DK119886.


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Study IDST001294
Study TitleEstimating Platelet Mitochondrial Function in Patients with Sepsis - Platelet NMRs (part-I)
Study Typesingle timepoint
Study SummaryRelationships between platelet mitochondrial oxygen consumption rates (mOCR) and metabolites in platelets as measured by quantitative 1H-NMR metabolomics. Samples collected in ED at a single timepoint. WB and platelets isolated from the same blood samples. Comparison of mitochondrial function and metabolomics in patients with sepsis and non-sepsis ED patients
University of Michigan; University of Mississippi; University of Minnesota
DepartmentClinical Pharmacy (UMich); Emergency Medicine (UMiss)
LaboratoryStringer NMR Metabolomics Laboratory
Last NameMcHugh
First NameCora
Address428 Church St, Ann Arbor, MI, 48103, USA
Submit Date2019-12-13
Num Groups2
Total Subjects23
Num Males12
Num Females11
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2020-05-26
Release Version1
Cora McHugh Cora McHugh application/zip

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Sample Preparation:

Sampleprep ID:SP001376
Sampleprep Summary:Methanol chlorform extraction of platelet pellet after 2 freeze-thaw cycles in the presence of methanol.
Sampleprep Protocol ID:Platelet Pellet MeOHCHCl3 Extraction
Sampleprep Protocol Filename:233.2.1_-_HAPS_Platelet_Pellet_MeOHCHCl3_Extraction
Processing Storage Conditions:On ice
Extraction Method:Methanol chloroform extraction
Extract Enrichment:Lyophilization
Extract Storage:-80℃
Sample Resuspension:500uL 50mM sodium phosphate buffer in D2O
Sample Spiking:4.99 mM DSS internal standard