Summary of Study ST001335
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000912. The data can be accessed directly via it's Project DOI: 10.21228/M8T112 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001335 |
Study Title | Air Pollution, Placenta Function, and Birth Outcomes in Los Angeles in the Placental Assessment in Response to ENvironmenTal pollution study (PARENTs) cohort |
Study Summary | This project aims to evaluate the internal environmental exposome of mother/fetus pairs within the PARENTs cohort, assessing a wide range of biomarkers/internal exposure measures for environmental toxins including air pollutants. Focusing on advanced imaging data on placental development and robust, clinically-confirmed birth outcomes, this a hypothesis-generating, untargeted pilot metabolomics study aims to identify potential predictors of placental insufficiencies and adverse birth outcomes. Measurements of exposures which include residential, occupational, and behavioral exposures, along with personalized air pollution measures, will help us in identifying related metabolomics patterns. |
Institute | Emory University |
Department | School of Medicine |
Laboratory | Clincal Biomarkers Laboratory |
Last Name | Uppal |
First Name | Karan |
Address | 615 Michael St. Ste 225, Atlanta, GA, 30322, USA |
kuppal2@emory.edu | |
Phone | (404) 727 5027 |
Submit Date | 2020-02-20 |
Total Subjects | 440 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Chear Study | Yes |
Analysis Type Detail | LC-MS |
Release Date | 2022-09-27 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP001417 |
Sampleprep Summary: | Samples were prepared for metabolomics analysis using established methods(Johnson et al. (2010). Analyst; Go et al. (2015). Tox Sci). Prior to analysis, plasma aliquots were removed from storage at -80 degrees C and thawed on ice. Each cryotube was then vortexed briefly to ensure homogeneity, and 50 microliters was transferred to a clean microfuge tube. Immediately after, the plasma was treated with 100 microliters of ice-cold LC-MS grade acetonitrile (Sigma Aldrich) containing 2.5 microliters of internal standard solution with eight stable isotopic chemicals selected to cover a range of chemical properties. Following addition of acetonitrile, urine was equilibrated for 30 min on ice, upon which precipitated proteins were removed by centrifuge (14,000 rpm at 4 degrees C for 10 min). The resulting supernatant (100 microliters) was removed, added to a low volume autosampler vial and maintained at 4 degrees C until analysis (<22 h). |
Sampleprep Protocol ID: | HRM_SP_082016_01 |
Sampleprep Protocol Filename: | EmoryUniversity_HRM_SP_082016_01.pdf |
Sampleprep Protocol Comments: | Date effective: 30 July 2016 |
Extraction Method: | 2:1 acetonitrile: sample followed by vortexing and centrifugation |