Summary of Study ST001345
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000914. The data can be accessed directly via it's Project DOI: 10.21228/M8JH69 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001345 |
| Study Title | C56BL6 WT or IFNARKO BMDM stimulated with different TLRs (part-V) |
| Study Summary | WT control or IFNAR deficient bone marrow derived macrophages were stimulated with IFNb, TLR3, IFNg, TLR4, and TLR4+IFNg ligands for 48 h. |
| Institute | University of California, Los Angeles |
| Last Name | Bensinger |
| First Name | Steven |
| Address | University of California, Los Angeles |
| bensingerlab@gmail.com | |
| Phone | 3102061016 |
| Submit Date | 2020-04-01 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | MS(Dir. Inf.) |
| Release Date | 2020-07-07 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP001427 |
| Sampleprep Summary: | A modified Bligh and Dyer extraction (Bligh and Dyer, 1959) was carried out on samples. Prior to biphasic extraction, a 13-lipid class Lipidyzer Internal Standard Mix is added to each sample (AB Sciex, 5040156). Following two successive extractions, pooled organic layers were dried down in a Genevac EZ-2 Elite. Lipid samples were resuspended in 1:1 methanol/dichloromethane with 10 mM Ammonium Acetate and transferred to robovials (Thermo 10800107) for analysis. |
