Summary of Study ST001346
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000917. The data can be accessed directly via it's Project DOI: 10.21228/M8568J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001346 |
| Study Title | Metabolomic changes in mouse liver on a protein restricted diet (part-I) |
| Study Summary | The data provided here are in support of the publication “Restriction of essential amino acids dictates the systemic metabolic response to dietary protein dilution” Yann W. Yap et al. Nature Communications 2020 (accepted for publication). Here we provide untargeted metabolomics LC-MS data from liver from C57Bl/6NCrl mice fed a diet in which dietary protein was restricted and corresponding unrestricted controls. Specifically, liver from animals on a low-protein diet following a week of diet adaptation and correspond controls with n = 5 for each group. |
| Institute | Monash University |
| Department | Department of Biochemistry and Molecular Biology |
| Laboratory | Nutrient Metabolism and Signalling Laboratory |
| Last Name | Rose |
| First Name | Adam |
| Address | Monash University, Clayton Campus, 23 Innovation Walk, Clayton, Victoria, 3800, Australia |
| adam.rose@monash.edu | |
| Phone | +61 3 99029340 |
| Submit Date | 2020-04-01 |
| Num Groups | 2 |
| Total Subjects | 10 |
| Num Males | 10 |
| Study Comments | Study 1 of 3 for this project |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-07-20 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP001428 |
| Sampleprep Summary: | Livers were cryogenically pulverized (cryopulverization) using a 12-well biopulverizer (BioSpec Products, OK USA Part number 59012MS) according to the manufacturer’s instructions. The frozen tissue powder was then weighed and extracted in 20 µL of extraction solvent (0°C) per mg of tissue. The mixture was then briefly vortexed before sonication in an ice-water bath for 10 minutes followed by centrifugation (20,000 rcf, 4℃, 10 minutes). The supernatant was then transferred to a mass spectrometry vial for LC-MS analysis. The extraction solvent consisted of 2:6:1 CHCl3:MeOH:H2O v/v/v with 2 µM CHAPS, CAPS, PIPES and TRIS as internal standards. |
| Processing Storage Conditions: | Described in summary |
| Extract Storage: | -80℃ |
