Summary of study ST001494

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001012. The data can be accessed directly via it's Project DOI: 10.21228/M8W99G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Show all samples  |  Perform analysis on untargeted data  |  Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data
Study IDST001494
Study TitleMetabolomics of murine embryos cultured in a microfluidic device and comparison with traditional microdrops culture
Study SummaryGlobal untargeted metabolomics study to analyse culture media extracted from an innovative microfluidic device or traditional microdrops in presence or absence of murine embryos to investigate PDMS-release of biomolecules and embryo metabolic activity.
Institute
University of Leeds
Last NameMancini
First NameVanessa
AddressWoodhouse Lane, Leeds, LS29JT
Emailelvm@leeds.ac.uk
Phone+447599197366
Submit Date2020-08-29
Analysis Type DetailLC-MS
Release Date2020-10-13
Release Version1
Vanessa Mancini Vanessa Mancini
https://dx.doi.org/10.21228/M8W99G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Sample Preparation:

Sampleprep ID:SP001576
Sampleprep Summary:Culture media samples (100 µl) were thawed on ice and prepared using previously described methods. Briefly, 300 µL of dry ice cooled methanol was added to individual culture medium samples and incubated overnight at -80°C; individual samples were spun down to remove proteins and subsequent supernatant used for analyses. Samples were separated and analysed using reverse-phase liquid chromatography connected to a a Thermo Scientific Q Exactive HF (LC-Hybrid Quadrupole-Orbitrap MS/MS) instrument using positive ion mode MS.
  logo