Summary of Study ST001508
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001017. The data can be accessed directly via it's Project DOI: 10.21228/M87M4S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001508 |
Study Title | Liver metabolomics in old liver-specific SIRT6 overexpressing mice |
Study Summary | Our previous data had suggested that gluconeogenesis capacity from the precursors lactate and glycerol declines in old C57BL/6 mice. This decline was rescued by whole body SIRT6 overexpression, but not in liver-specific SIRT6 overexpression. Here we preformed liver metabolomics in old liver-specific SIRT6 overexpression and control mice. |
Institute | Bar Ilan University |
Department | The Mina & Everard Goodman Faculty of Life Sciences |
Laboratory | Laboratory of Prof. Haim Cohen |
Last Name | Cohen |
First Name | Chaim |
Address | Bar Ilan University, Ramat Gan, Israel, 5290002 |
Haim.Cohen@biu.ac.il | |
Phone | +97235318383 |
Submit Date | 2020-10-07 |
Num Groups | 4 |
Total Subjects | 20 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2021-03-11 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001590 |
Sampleprep Summary: | Frozen liver pieces weighting ~30mg were transferred into soft tissue homogenizing CK 14 tubes containing 1.4 mm ceramic beads (Bertin corp.) prefilled with 1 ml of cold (-20°C) metabolites extraction solvent (Methanol: Acetonitrile:H2O, 50:30:20) and kept on ice. Samples were homogenized using precellys 24 tissue homogenizer (3X20sec at 6000 rpm, with a 30sec gap between each of the three cycles, Bertin Technologies) cooled to 2°C. Homogenized extracts were centrifuged in the Precellys tubes at 18,000g for 15 minutes at 4°C, supernatants were collected in microcentrifuge tubes and centrifuged again at 18,000g for 10 minutes at 4°C. The supernatants were transferred to glass HPLC vials and kept at -75 °C prior to LC-MS analysis. |
Extract Storage: | -80℃ |