Summary of Study ST001632

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001043. The data can be accessed directly via it's Project DOI: 10.21228/M8W41Q This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST001632
Study Title	Lipidomics of Ginseng Total Secondary Saponins on Myocardial Ischemia Rat
Study Summary	Cardioprotective Effect of Ginseng Total Secondary Saponins on Myocardial Ischemia Rat via Regulating Disturbed Lipids Based on Lipidomics.
Institute	Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College
Last Name	Wang
First Name	Zhe
Address	No.2 nanwei street, xichen district, Beijing, China
Email	wangzhe@imm.ac.cn
Phone	+861083154880
Submit Date	2020-11-22
Raw Data Available	Yes
Raw Data File Type(s)	d
Analysis Type Detail	LC-MS
Release Date	2023-01-02
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP001715
Sampleprep Summary:	100μl of plasma or tissue homogenate was transferred into a 10 ml glass tube and added 20 μl of lipid internal standards. After vortexed for 10 s, 1.5 ml of methanol and 5 ml methyl tert-butyl ether were added to the tube, and the sample was vortexed for 15 min and then added 1.5 ml water, then centrifuged at 4500r/min for 10 min (4 ℃). The upper organic phase was transferred to another glass tube, and the lower water phase was added with 2 ml secondary extractant (methyl tert-butyl ether /methanol/water,10:3:2.5, v/v/v). After vortexed for 10min and centrifuged for 15min at 4500rpm, and the upper organic phase was combined and dried under a gentle nitrogen stream. The residue was re-dissolved in 100 μl methanol/chloroform (1:1, v/v).

Sampleprep ID:

SP001715

Sampleprep Summary:

100μl of plasma or tissue homogenate was transferred into a 10 ml glass tube and added 20 μl of lipid internal standards. After vortexed for 10 s, 1.5 ml of methanol and 5 ml methyl tert-butyl ether were added to the tube, and the sample was vortexed for 15 min and then added 1.5 ml water, then centrifuged at 4500r/min for 10 min (4 ℃). The upper organic phase was transferred to another glass tube, and the lower water phase was added with 2 ml secondary extractant (methyl tert-butyl ether /methanol/water,10:3:2.5, v/v/v). After vortexed for 10min and centrifuged for 15min at 4500rpm, and the upper organic phase was combined and dried under a gentle nitrogen stream. The residue was re-dissolved in 100 μl methanol/chloroform (1:1, v/v).