Summary of Study ST001639

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001048. The data can be accessed directly via it's Project DOI: 10.21228/M87D6G This work is supported by NIH grant, U2C- DK119886.


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Study IDST001639
Study TitlePlasma Metabolomic signatures of COPD in a SPIROMICS cohort
Study SummaryThe Subpopulations and Intermediate Outcome Measures in COPD Study (SPIROMICS) ( Identifier: NCT01969344) includes 2,771 subjects, aged 40-80 years with at least 20 pack-years of smoking. An additional 202 subjects were never smokers. Fasting blood drawn at the enrollment visit using a p100 tube. The first 649 subjects who returned for a 5-7 year visit (Visit 5) were selected for this study. The blood profiled were from the year 1 visit.
National Jewish Health
DepartmentDivision of Pulmonary Medicine
Last NameBowler
First NameRussell
Address1400 Jackson St. Denver, CO 80206
Phone303 270 2014
Submit Date2020-12-30
Total Subjects649
Num Males350
Num Females299
Analysis Type DetailLC-MS
Release Date2021-05-30
Release Version1
Russell Bowler Russell Bowler application/zip

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Sample Preparation:

Sampleprep ID:SP001722
Sampleprep Summary:Following receipt, samples were inventoried and immediately stored at -80oC. Each sample received was accessioned into the Metabolon LIMS system and was assigned by the LIMS a unique identifier that was associated with the original source identifier only. This identifier was used to track all sample handling, tasks, results, etc. The samples (and all derived aliquots) were tracked by the LIMS system. All portions of any sample were automatically assigned their own unique identifiers by the LIMS when a new task was created; the relationship of these samples was also tracked. All samples were maintained at -80oC until processed. Samples were prepared using the automated MicroLab STARĀ® system from Hamilton Company. Several recovery standards were added prior to the first step in the extraction process for QC purposes. To remove protein, dissociate small molecules bound to protein or trapped in the precipitated protein matrix, and to recover chemically diverse metabolites, proteins were precipitated with methanol under vigorous shaking for 2 min (Glen Mills GenoGrinder 2000) followed by centrifugation. The resulting extract was divided into five fractions: two for analysis by two separate reverse phase (RP)/UPLC-MS/MS methods with positive ion mode electrospray ionization (ESI), one for analysis by RP/UPLC-MS/MS with negative ion mode ESI, one for analysis by HILIC/UPLC-MS/MS with negative ion mode ESI, and one sample was reserved for backup. Samples were placed briefly on a TurboVapĀ® (Zymark) to remove the organic solvent. The sample extracts were stored overnight under nitrogen before preparation for analysis.