Summary of Study ST001645
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000747. The data can be accessed directly via it's Project DOI: 10.21228/M8469M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001645 |
| Study Title | Variability in metabolomic profiles among unique genotypes of Acropora cervicornis (part -III) |
| Study Type | intraspecific variability |
| Study Summary | This project aims to identify differences in metabolomic profiles among seven known, unique genotypes of the threatened staghorn coral Acropora cervicornis. |
| Institute | University of Florida |
| Department | SECIM |
| Last Name | Patterson |
| First Name | Joshua |
| Address | Florida Aquarium Center for Conservation, 529 Estuary Shore Lane, Apollo Beach, FL 33572 |
| joshpatterson@ufl.edu | |
| Phone | (813) 419-4917 |
| Submit Date | 2020-12-22 |
| Num Groups | 7 |
| Total Subjects | 41 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2021-06-22 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP001728 |
| Sampleprep Summary: | Metabolomic analyses were performed at the Southeast Center for Integrated Metabolomics (SECIM) at the University of Florida. Dried powder of aqueous phase samples acquired from methanol/chloroform extraction were dissolved in 50mM sodium phosphate buffer with 0.5mM D6-deuterated sodium trimethylsilylpropanesulfonate (DSS-d6). NMR spectra were measured using the first slice of a NOESY pulse sequence (tnnoesy) using 14.1 T Bruker Avance II NMR system with a CP TXI CryoProbe. The acquisition parameters used in Lohr et al. (2019) and Myer et al. (2020) were utilized to acquire proton spectra. All spectra were processed and the integrated area was extracted using MestReNova 11.0-17609 (Mestrelab Research S.L.). Before Fourier transformation, baseline correction and phase correction were applied with a line-broadening factor of 0.22 Hz and spectra were normalized with respect to a DSS signal at 0.0 ppm. |
| Processing Method: | Lyophilization and Homogenization |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | Modified FOLCH extraction |
| Extract Storage: | -80℃ |
| Sample Resuspension: | In 50 mM phosphate buffer (pH 7.2) with 2 mM EDTA, 0.5 mM DSS and 0.2% sodium azide for aqueous phase samples. |
| Sample Spiking: | 0.5 mM of DSS for aqueous phase samples |
