Summary of Study ST001668

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001071. The data can be accessed directly via it's Project DOI: 10.21228/M8870S This work is supported by NIH grant, U2C- DK119886.

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Study IDST001668
Study TitleD-Allulose effects on hepatic metabolomics profile in rodents
Study SummaryTo determine the hepatic metabolomics profile after D-allulose intake.
Institute
Matsutani Chemical Industry Co., Ltd.
Last NameKanasaki
First NameAkane
Address5-3 Kita-Itami, Itami, Hyogo, 664-8508, Japan
Emailakane-kanasaki@matsutani.co.jp
Phone81-72-771-2052
Submit Date2021-01-19
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2022-01-19
Release Version1
Akane Kanasaki Akane Kanasaki
https://dx.doi.org/10.21228/M8870S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP001751
Sampleprep Summary:For CE-TOFMS, The frozen tissue was plunged into 50% acetonitrile/Milli-Q water containing internal standards. The tissues were homogenized thrice, and then centrifuged. The upper aqueous layer was centrifugally filtered to remove the proteins. The filtrate was then centrifugally concentrated and re-suspended in 50 µL of Milli-Q water for CE-MS analysis. For LC-TOFMS, the frozen tissue was plunged into 1% formic acid/acetonitrile containing the internal standard solution. Tissue was homogenized thrice, and the mixture was yet again homogenized after adding Milli-Q water and then centrifuged. The supernatant was collected and 500 1% formic acid/acetonitrile and Milli-Q water were added to the precipitate. Homogenization and centrifugation were performed as described previously, and the supernatant was mixed with previously collected one. The mixed supernatant was filtrated to remove the proteins and phospholipids. The filtrate was then desiccated and dissolved in 200 µL of isopropanol/Milli-Q water for LC-MS analysis.
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