Summary of Study ST001676
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001078. The data can be accessed directly via it's Project DOI: 10.21228/M8C111 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001676 |
| Study Title | Lipidomic analysis of CD4+ T-cell subsets (Th1,Th2,Th17 and iTreg cells) (part I) |
| Study Type | MS, untargeted cell-based lipidomics |
| Study Summary | Part 1/5: It includes lipidomic analysis of CD4+ T-cell subsets(Th1,Th2,Th17 and iTreg cells)and their paired controls(Th0 cells). |
| Institute | University of Turku |
| Department | Systems Medicine, Turku Bioscience |
| Laboratory | Systems Medicine |
| Last Name | Sen |
| First Name | Partho |
| Address | Tykistökatu 6B, BioCity, 5th Floor, Turku, Southwest, 20521, Finland |
| partho.sen@utu.fi | |
| Phone | 0469608145 |
| Submit Date | 2021-01-28 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-11-02 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP001759 |
| Sampleprep Summary: | The samples were randomized and extracted using a modified version of the previously-published Folch procedure. Briefly, 150 µL of 0.9% NaCl was added to cell pellets, and samples then vortexed and ultrasonicated for 3 minutes. Next, 20 µL of the cell suspension was mixed with 150 µL of the 2.5 µg mL-1 internal standards solution in ice-cold CHCl3:MeOH (2:1, v/v). The internal standard solution contained the following compounds: 1,2-diheptadecanoyl-sn-glycero-3-phosphoethanolamine (PE (17:0/17:0)), N-heptadecanoyl-D-erythro-sphingosylphosphorylcholine (SM(d18:1/17:0)), N-heptadecanoyl-D-erythro-sphingosine (Cer(d18:1/17:0)), 1,2-diheptadeca-noyl-sn-glycero-3-phosphocholine (PC(17:0/17:0)), 1-heptadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine (LPC(17:0)) and 1-palmitoyl-d31-2-oleoyl-sn-glycero-3-phosphocholine (PC(16:0/d31/18:1)). These were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). In addition, triheptadecanoin (TG(17:0/17:0/17:0)) was purchased from (Larodan AB, (Solna, Sweden). The samples were vortexed and incubated on ice for 30 min after which they were centrifuged at 7800 × g for 5 min. Finally, 60 µL from the lower layer of each sample was collected and mixed with 60 µL of ice cold CHCl3:MeOH (2:1, v/v) in LC vial. The total protein content in cells was measured by the Bradford method. |
| Sampleprep Protocol Filename: | parthosen_20210128_003828_PR_CO_Cell_Prep_Protocol.pdf |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -80℃ |
