Summary of Study ST001852
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001168. The data can be accessed directly via it's Project DOI: 10.21228/M8QT2F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001852 |
Study Title | Screening of unique bile acid metabolizing bacteria |
Study Summary | We incubated individual bacterial strains at pH 7 or pH 9 with either CDCA, LCA, or 3-oxo-Δ4-LCA as starting substrates. Culture supernatants were collected after 48 hours and 14 bile acids were measured by targeted metabolomics. |
Institute | Keio University School of Medicine |
Department | Dept of Microbiology and Immunology |
Last Name | Koji |
First Name | Atarashi |
Address | 35 Shinanomachi, Shinjuku-ku, Tokyo, JAPAN |
kojiatarashi@keio.jp | |
Phone | 0353633769 |
Submit Date | 2021-06-24 |
Raw Data Available | Yes |
Raw Data File Type(s) | lcd |
Analysis Type Detail | LC-MS |
Release Date | 2021-07-07 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP001935 |
Sampleprep Summary: | 100 μL of culture supernatant was transferred into a screw-cap glass vial containing 10 μL of deuterium-labelled internal standards (d4-CA, d4-CDCA, and d4-LCA, 1 nmol/mL each). 400 μL of water was added and sonicated for 10 min, then applied onto the solid-phase extraction cartridge (Agilent Bond Elut C18, 100 mg/1 mL, preconditioned with 1 mL of methanol and 3 mL of water). The cartridge was washed with 1 mL of water and captured bile acids were eluted with 1 mL of 90% ethanol. After solvent evaporation, the remaining residue was dissolved in 100 μL of 50% ethanol |