Summary of Study ST001913
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000319. The data can be accessed directly via it's Project DOI: 10.21228/M8H029 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001913 |
| Study Title | Stool metabolomics in the New Hampshire Birth Cohort Study |
| Study Type | Untargeted and semi-targeted metabolomics analysis |
| Study Summary | This is a study of data collected from fecal samples from larger New Hampshire Birth Cohort Study (NHBCS). 1H NMR metabolomic profiling of 524 infant stool samples collected at 6 week - 3 year age was performed and spectra were binned (untargeted metabolomics). A set of host-microbiome co-metabolites were library matched in individual sample spectra and their relative concentrations were determined. This study investigated associations of the functional metabolic response of the microbial milieu of the infant gut with environmental and other factors. |
| Institute | University of North Carolina at Chapel Hill |
| Department | Nutrition |
| Laboratory | Metabolomics and Exposome Laboratory, Nutrition Research Institute, UNC Chapel Hill |
| Last Name | Sumner |
| First Name | Susan |
| Address | 500 Laureate Way, Kannapolis, NC 28081 |
| susan_sumner@unc.edu | |
| Phone | (919) 622-4456 |
| Submit Date | 2021-08-09 |
| Num Groups | 2 |
| Total Subjects | 524 |
| Study Comments | The number of groups includes the QC samples, Total samples = 572 |
| Raw Data Available | Yes |
| Analysis Type Detail | NMR |
| Release Date | 2023-01-31 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP001997 |
| Sampleprep Summary: | De-identified stool aliquots were shipped on dry ice and immediately stored at -80 °C for metabolomics analysis. Samples were randomized into batches. For each batch, samples were thawed and ~150mg of stool was transferred to MagNA Lyser tubes after recording the weight, Samples were then homogenized with 50% acetonitrile in water by using the Omni Bead Disruptor (Omni International, GA, USA). Homogenized samples were centrifuged at 16000 rcf and the supernatant was separated into another tube. An aliquot (1000 uL, 100 mg equivalent of fecal mass) was transferred into Eppendorf tube and lyophilized overnight. The dried extract was reconstituted in 700 uL of NMR master mix (containing 0.2M phosphate, 0.5 mM DSS-d6, and 0.2% sodium azide), vortexed on a multitube vortexer at speed 5 for 2 min and centrifuged at 16000 rcf for 5 min. A 600 µl aliquot of the supernatant was transferred into a pre-labeled 5mm NMR tube for data acquisition on a 700 MHz spectrometer. Additionally, study pooled samples (created from randomly selected study samples) and batch pooled quality control (QC) samples were generated from supernatants of study samples and aliquots of supernatants were dried and reconstituted similar to study samples described above, and used for QC purposes. |
| Sampleprep Protocol Filename: | 1. Dartmouth-Fecal Metabolomics_NMR_Batch2 Procedures |
| Processing Storage Conditions: | 4℃ |
| Extraction Method: | 50% Acetonitrile in Water |
| Extract Storage: | -80℃ |
| Sample Resuspension: | In phosphate buffer containing D2O |
