Summary of Study ST001955

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001242. The data can be accessed directly via it's Project DOI: 10.21228/M85H78 This work is supported by NIH grant, U2C- DK119886.


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Study IDST001955
Study TitleMetabonomics analysis reveals the physiological mechanism of promoting maize shoots growth under negative pressure to stabilize soil water content
Study SummaryThe purpose of this study is to analyze maize shoots growth under negative pressure to stabilize soil water content,Maize plants were subjected to two irrigation treatments. The first treatment was soil moisture dry-wet cycles, which was obtained using drip irrigation (control, DW). The second treatment was negative pressure to stabilize soil water content treatment (SW), which was obtained using the negative pressure irrigation (NPI) system.
Heilongjiang Bayi Agricultural University
Last NameZhang
First NameJili
AddressHigh tech Zone, Longfei District, Daqing, Heilongjiang, 163319, China
Submit Date2021-10-03
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailAPCI-MS
Release Date2021-12-15
Release Version1
Jili Zhang Jili Zhang application/zip

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Sample Preparation:

Sampleprep ID:SP002039
Sampleprep Summary:Collected samples were thawed on ice, and metabolites were extracted from 20 µL of each sample using 120 µL of precooled 50% methanol buffer. Next, the mixture of metabolites was vortexed for 1 min, followed by incubation for 10 min at room temperature, and storing at -20°C overnight. On the next day, the mixture was centrifuged at 4,000 g for 20 min and the supernatant was subsequently transferred to 96 well plates. Samples were stored at ‐80°C prior to the LC‐MS analysis. Pooled quality control (QC) samples were also prepared by combining 10 μL of each extraction mixture