Summary of Study ST001956
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001243. The data can be accessed directly via it's Project DOI: 10.21228/M81T40 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001956 |
| Study Title | Timecourse exometabolome analysis of glucose grown Rubrivivax benzoatilyticus cells |
| Study Type | Timecourse experiment |
| Study Summary | Bacterial cells were grown on glucose under photoheterotrophic conditions for 18 days. Spent media of cells, harvested at 3rd, 9th and 18th day of growth, was vacuum dried and the metabolome was extracted in methanol. The extracted metabolites were derivatized and analyzed using GC-MS. |
| Institute | University of Hyderabad |
| Last Name | Gupta |
| First Name | Deepshikha |
| Address | Dept. of Plant Sciences, |
| deepshikha@uohyd.ac.in | |
| Phone | +918985420802 |
| Submit Date | 2021-10-13 |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-10-31 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP002042 |
| Sampleprep Summary: | metabolites were extracted from dried culture supernatant in 1 ml methanol. Methanol extract (100 µl) was vacuum dried. The dried exometabolome samples were derivatized, to protect their functional groups, with 40 µl BSTFA (N,O-bis(trimethylsilyl)trifluoroacetamide) and TMCS (trimethylchlorosilane); incubated at 70 ○C for 4 h in dry bath and immediately analysed by GC-MS. |
| Processing Storage Conditions: | -20℃ |
