Summary of Study ST002076
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001316. The data can be accessed directly via it's Project DOI: 10.21228/M8M990 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002076 |
Study Title | Metabolomic study of Escherichia coli K-12 MG1655 WT and its transcriptional regulator double mutants under anaerobic fermentation conditions |
Study Type | Metabolomics |
Study Summary | Metabolomic analysis of Wildtype, fnr_arcA (FA), arcA_ihf (AI) and fnr_ihf (FI) mutants in glucose minimal media under anaerobic fermentation conditions during its exponential phase of growth. Three biological and two technical replicate samples (n=6) were harvested for each of the strains while growing in a bioreactor anaerobically at 37 degrees Celsius and 150 rpm. This study aims to characterize and compare the metabolic profiles of all these strains. |
Institute | IIT Bombay |
Department | Department of Chemical Engineering |
Laboratory | Systems Biology and Metabolic Engineering Laboratory (SBMEL) |
Last Name | Pal |
First Name | Ankita |
Address | Department of Chemical Engineering, IIT Bombay, Mumbai, Maharashtra, 400076, India |
pal.ankita90@gmail.com | |
Phone | 08879536816 |
Submit Date | 2022-02-02 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2022-06-30 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP002165 |
Sampleprep Summary: | Samples were extracted from the Wildtype, FA, AI and FI mutants grown in a bioreactor anaerobically in glucose minimal media at 37 degrees Celsius and 150 rpm. The metabolites were extracted using the Methanol-Chloroform-Water method. The samples were spiked with an equal volume of 13C-labelled internal standard taken from the same batch at an earlier stage to prevent to account for losses due to metabolite degradation and to enable robust quantification circumventing the ion-suppression effects. |
Sampleprep Protocol Filename: | Method_msi.pdf |