Summary of Study ST002078
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001318. The data can be accessed directly via it's Project DOI: 10.21228/M8BT55 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002078 |
| Study Title | Multiple modes of interfering with the activity of Plasmodium falciparum cytoplasmic isoleucyl-tRNA synthetase illustrate the enzyme is a promising antimalarial target. |
| Study Summary | Here we interrogated the in vitro metabolic effects of 6 drugs using ultra-high performance liquid chromatography mass-spectrometry (UHPLC-MS). The resulting metabolic fingerprints provide information on the parasite biochemical pathways affected by pharmacologic intervention. We interrogate the metabolic effects on the 3D7 parasite strain. The metabolic effects were compared to their parent strain. The metabolic fingerprints provided show that certain biochemical pathways are affected by the drug inhibitory effect. |
| Institute | Pennsylvania State University |
| Department | Department of Biochemistry and Molecular Biology |
| Last Name | Llinas |
| First Name | Manuel |
| Address | W126 Millenium Science Complex, University Park, PA 16802 |
| manuel@psu.edu | |
| Phone | 814-867-3527 |
| Submit Date | 2021-12-06 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-10-19 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP002167 |
| Sampleprep Summary: | 90% Methanol/10% water at 50:1 solvent/cell pellet followed by vortexing and clarification via centrifugation |
| Processing Method: | Lysis |
| Processing Storage Conditions: | 4℃ |
| Extraction Method: | See SA Cobbold et al JBC 2013 |
| Extract Cleanup: | Clarification via centrifugation followed by nitrogen drying |
| Extract Storage: | -80℃ |
| Sample Spiking: | Internal Standard 13C4, 15N1-Aspartate used for sample preparation normalizer |
