Summary of Study ST002154

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001367. The data can be accessed directly via it's Project DOI: 10.21228/M8170M This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST002154
Study Title	Virus-host interaction studies of P. plecoglossicida strain (NZBD9) and Epinephelus coioides.
Study Summary	E. coioides of the infection group were intrapleurally injected with 105 colony-forming units per fish (cfu/fish) of P. plecoglossicida (wild-type strain or clpV-RNAi strain), while control E. coioides received an equivalent volume of phosphate-buffered saline (PBS). The temperature of the water was maintained at 18 ± 1 °C during the infection experiment. The spleens of E. coioides were collected at 6, 12, 24, 48, 72 and 96 hours post injection (hpi).
Institute	Jimei University
Last Name	Yang
First Name	Zhiqiang
Address	No. 185 Yinjiang Road, Jimei District, Xiamen City, Fujian Province, No. 185 Yinjiang Road
Email	201911832016@jmu.edu.cn
Phone	5926181487
Submit Date	2022-04-09
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2022-05-16
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002246
Sampleprep Summary:	The deep-frozen spleen tissue in each replicate was accurately weighed (about 15 mg) and then transferred to an Eppendorf tube. Each sample was first spiked with 300 μL of methanol containing internal standards and then homogenized at 65 Hz for 2.5 min. The homogenate was mixed with 1 mL of MTBE, and thoroughly vortexed for 30 min. Then, 300 μL of Milli-Q water was added to the tube, followed by vortexing for 1 min and centrifugation for 10 min to form a two-phase system (12,000 rpm, 4 ℃). Each specimen was prepared by mixing 250 μL of the top layer plus 150 μL of the bottom layer accurately and then vacuum-dried in a Speedvac concentrator (Thermo Scientific, USA).

Sampleprep ID:

SP002246

Sampleprep Summary:

The deep-frozen spleen tissue in each replicate was accurately weighed (about 15 mg) and then transferred to an Eppendorf tube. Each sample was first spiked with 300 μL of methanol containing internal standards and then homogenized at 65 Hz for 2.5 min. The homogenate was mixed with 1 mL of MTBE, and thoroughly vortexed for 30 min. Then, 300 μL of Milli-Q water was added to the tube, followed by vortexing for 1 min and centrifugation for 10 min to form a two-phase system (12,000 rpm, 4 ℃). Each specimen was prepared by mixing 250 μL of the top layer plus 150 μL of the bottom layer accurately and then vacuum-dried in a Speedvac concentrator (Thermo Scientific, USA).