Summary of Study ST002287

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001467. The data can be accessed directly via it's Project DOI: 10.21228/M83H68 This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002287
Study TitleEnhanced systemic commensal E. coli immunogenicity through minor alteration of the metabolic profile
Study SummaryLC-MS data from the supernatant and cell pellet of cultured commensal E. coli with and without mutations in outer membrane porin C
University of Calgary
DepartmentMicrobiology and Infectious Disease
Last NameBrown
First NameKirsty
AddressHSC 1729, 3330 Hospital Dr NW
Submit Date2022-09-26
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2023-05-04
Release Version1
Kirsty Brown Kirsty Brown application/zip

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Sample Preparation:

Sampleprep ID:SP002379
Sampleprep Summary:Metabolites from supernatant were extracted in 100% methanol, precipitated at -20°C for 1 hour, centrifuged and diluted into a linear range (1:20 final dilution) with 50% methanol. To extract intracellular metabolites, bacterial pellet was snap-frozen in liquid nitrogen, resuspended in cell lysis buffer (1M Tris, 5M NaCL, 0.5M EDTA, 10% SDS in H2O) and sonicated. Metabolites were extracted as described above