Summary of Study ST002287
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001467. The data can be accessed directly via it's Project DOI: 10.21228/M83H68 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002287 |
| Study Title | Enhanced systemic commensal E. coli immunogenicity through minor alteration of the metabolic profile |
| Study Summary | LC-MS data from the supernatant and cell pellet of cultured commensal E. coli with and without mutations in outer membrane porin C |
| Institute | University of Calgary |
| Department | Microbiology and Infectious Disease |
| Laboratory | Geuking |
| Last Name | Brown |
| First Name | Kirsty |
| Address | HSC 1729, 3330 Hospital Dr NW |
| kirsty.brown1@ucalgary.ca | |
| Phone | 2508692232 |
| Submit Date | 2022-09-26 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-05-04 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP002379 |
| Sampleprep Summary: | Metabolites from supernatant were extracted in 100% methanol, precipitated at -20°C for 1 hour, centrifuged and diluted into a linear range (1:20 final dilution) with 50% methanol. To extract intracellular metabolites, bacterial pellet was snap-frozen in liquid nitrogen, resuspended in cell lysis buffer (1M Tris, 5M NaCL, 0.5M EDTA, 10% SDS in H2O) and sonicated. Metabolites were extracted as described above |
