Summary of Study ST002366

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001521. The data can be accessed directly via it's Project DOI: 10.21228/M83707 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002366
Study TitleSingle cell lipidome analysis of phosphatidylcholines and spingomyelins from HepG2 and C2C12 cells
Study TypeQuantitative single cell lipidomics
Study SummaryWe have developed a high-throughput method for the detection and quantification of a wide range of phosphatidylcholine (PC) and sphingomyelin (SM) species from single cells that combines fluorescence-assisted cell sorting (FACS) with automated chip-based nanoelectrospray ionization (nanoESI) and shotgun lipidomics. Using this method we can detect and perform relative quantitation on more than >50 different PC and SM species from immortalised human cells, and can easily distinguish between cells of different lineages (e.g. hepatocarcinoma HePG2 vs C2C12 myoblasts) and cells treated with exogenous fatty acids.
Institute
Victor Chang Cardiac Research Institute
LaboratoryCellular Bioenergetics Laboratory
Last NameHancock
First NameSarah
AddressLowy Packer Building, 405 Liverpool Street, Darlinghurst, NSW, 2010, Australia
Emails.hancock@victorchang.edu.au
Phone+61414537526
Submit Date2022-10-19
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailMS(Dir. Inf.)
Release Date2023-10-19
Release Version1
Sarah Hancock Sarah Hancock
https://dx.doi.org/10.21228/M83707
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP002461
Sampleprep Summary:Samples were prepared one of three ways: as bulk extracts from ~1 million cells, or from fluorescence-assisted cell sorting (FACS) into either pooled fifty cells samples or singly-isolated cells. Full sample prep details are provided in the attached protocol.
Sampleprep Protocol Filename:MWB_sampleprep_protocol.pdf
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