Summary of Study ST002373
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001526. The data can be accessed directly via it's Project DOI: 10.21228/M8FH84 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002373 |
Study Title | Extracellular metabolome of activated CD8+ T cells |
Study Summary | WT OT-I CD8+ T cells were activated with plate-bound anti-CD3 and soluble anti-CD28. Blank media or media post 10hr, 30hr or 48hr cell culture were collected for mass spectrometry analysis. |
Institute | Johns Hopkins University |
Last Name | Xu |
First Name | Wei |
Address | 1650 Orleans Street, Baltimore, MD 21287, USA. |
wxu29@jhmi.edu | |
Phone | 443-220-9936 |
Submit Date | 2022-11-29 |
Raw Data Available | Yes |
Raw Data File Type(s) | wiff |
Analysis Type Detail | LC-MS |
Release Date | 2023-11-28 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP002468 |
Sampleprep Summary: | For measurement of extracellular metabolome, 625 µL of media was added to 375 µL acetonitrile ACN. Samples were stored at -20 °C for at least two hours followed by centrifugation at 14,000xg to precipitate any proteins. 250 µL of the supernatant was mixed with 250 µL of water and added to a 3 kDa molecular weight cut-off filter spin column (Microcon YM-3 Centrifugal Filter, Millipore). Samples were centrifuged at 14,000xg at 4 °C for 30 min. The flow-through was saved for LC-MS/MS analysis. |