Summary of Study ST002440
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001572. The data can be accessed directly via it's Project DOI: 10.21228/M8H70Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002440 |
| Study Title | Impacts of interactions between environmental chemical exposures and diet on gut microbiota and microbiota-derived metabolites in mothers and children (MAAP Cohort) |
| Study Type | Prospective Cohort Study |
| Study Summary | In 2014, the Microbes, Allergy, Asthma and Pets (MAAP) study (P01 AI089473) was launched. All women ages 18-49 years scheduled for prenatal visits at Henry Ford Health, expected to deliver during the recruitment period at a Henry Ford Health hospital, and whose residence was in a predefined area of Detroit and metropolitan Detroit suburbs were potentially eligible. There were no medical insurance requirements. Between 2014 and 2016, 141 women were enrolled; their infant was followed through age 18 months (with continuing follow-up through early/mid childhood underway through the NIH ECHO program under the CREW birth cohort consortium (5UG3OD023282, Gern PI). Blood and stool samples were obtained from the mother prenatally and stool specimens were obtained from the infant at 5 time points (1-week, 1-, 3-, 6- and 18-months) with 16S sequencing already completed. Blood was also obtained from the infant at pre-defined intervals. Please contact Andrea Cassidy-Bushrow at acassid1@hfhs.org for questions related to the subject characteristics and outcomes. This research was supported by the Environmental influences on Child Health Outcomes (ECHO) OIF program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. MAAP is an ECHO cohort which is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375 ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382 North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857 Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539 |
| Institute | Wayne State University |
| Department | Pharmacology |
| Last Name | Petriello |
| First Name | Michael |
| Address | 6135 Woodward Ave, Detroit, MI 48202 |
| michael.petriello@wayne.edu | |
| Phone | 313-577-1089 |
| Submit Date | 2023-01-12 |
| Total Subjects | 31 |
| Study Comments | ECHO Project EC0549, HHEAR Project E020-0012 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-01-12 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP002535 |
| Sampleprep Summary: | Study samples (500-1000 µL aliquots) were shipped from the Henry Ford Health System to the NC HHEAR Hub on dry ice. The NC HHEAR Hub thawed and aliquoted the study samples at 50 µL per aliquot. An additional 10 µL was taken from each study sample, mixed, and distributed at 50 µL per aliquot, which were used as QC study pools throughout the study. HHEAR reference plasma (50 µL each) and NIST plasma (1950) reference material (50 µL each) were provided by the NC HHEAR Hub. LC-MS grade water (50 µL) was used as blank. All samples were thawed at 4°C overnight before the preparation. Samples, including study samples, study pool samples, HHEAR reference plasma, NIST reference plasma, and blanks were mixed with 400 µL methanol containing 500 ng/mL tryptophan-d5 as internal standard and vortexed by a multiple tube vortex mixer for 2 min at 5000 rpm at room temperature. All samples were centrifuged at 16,000 rcf for 10 min at 4°C. The supernatant (350 µL) was transferred into a pre-labeled 2.0 mL Lo-bind Eppendorf tube, dried by a SpeedVac overnight, and stored at -80° C. For immediate analysis, 100 µL of water-methanol solution (95:5, v/v) was used to reconstitute the dried extracts. Samples were thoroughly mixed on a multiple tube vortex mixer for 10 min at 5000 rpm at room temperature and then centrifuged at 4°C for 10 min at 16,000 rcf. The supernatant was transferred to pre-labeled autosampler vials for data acquisition by LC-MS. |
| Processing Storage Conditions: | On ice |
| Extraction Method: | Vortex with methanol containing 500ng/ml tryptophan-d5 as internal standard |
| Extract Storage: | -80℃ |
| Sample Resuspension: | Water-Methanol (95:5, v/v) |
| Sample Spiking: | Tryptophan-d5 stock solution at 500 ng/mL |
