Summary of Study ST002452

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001581. The data can be accessed directly via it's Project DOI: 10.21228/M8BD85 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002452
Study Title	Lipidomic analysis of human brain from frontotemporal dementia cases of with GRN and C9orf72 mutations
Study Summary	Lipidomic analysis carried out on postmortem human brain tissue from cases with FTD carrying inherited mutations in the GRN gene, or repeat expansions in the C9orf72 gene, and age-matched control cases. Tissue was sampled from the heavily affected superior frontal grey and white matter, and less heavily affected superior parietal grey and white matter.
Institute	The University of Sydney
Last Name	Don
First Name	Anthony
Address	Office 3217, D17 Charles Perkins Centre, Camperdown, NSW, 2006, Australia
Email	anthony.don@sydney.edu.au
Phone	+61286275578
Submit Date	2023-01-19
Num Groups	3
Total Subjects	28
Num Males	13
Num Females	15
Raw Data Available	Yes
Raw Data File Type(s)	mzXML
Analysis Type Detail	LC-MS
Release Date	2023-03-01
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002552
Sampleprep Summary:	Lipids were extracted from 100uL of brain homogenate using a two-phase methyl-tert-butyl ether (MTBE)/methanol/water protocol as described by Matyash et al. (2008), with the addition of the following internal standards: 2000 pmoles SM(d18:1_12:0), 2000 pmoles GluCer(d18:1_12:0), 500 pmoles LacCer(d18:1_12:0), 500 pmoles ST(18:1_17:0), 500 pmoles Cer(18:1/17:0), 200 pmoles C17:0 Sph, 200 pmoles C17:1 S1P, 200 pmoles d3-C16 AcCa, 500 pmoles C17:1 LPE, 500 pmoles C17:1 LPS, 200 pmoles C17:0 LPA, 500 pmoles C17:0 LPC, 5000 pmoles PC(19:0_19:0), 2000 pmoles PS(17:0_17:0), 2000 pmoles C17:0 PE, 2000 pmoles C17:0 PG, 1000 pmoles C17:0 PA, 1000 pmoles d7-18:1_15:0 PI, 2000 pmoles CL(14:0_14:0_14:0_14:0), 2000 pmoles TAG(17:0_17:0_17:0), 500 pmoles DAG d7- 18:1/15:0), 500 pmoles d7- 18:1 MAG, 2000 pmoles C17:0 CholE, 1000 pmoles d7 Chol. Lipids were reconstituted in 400uL of 100% HPLC methanol, then diluted 1/5 in 80% v/v HPLC methanol, containing 1 mM ammonium formate and 0.2% formic acid.

Sampleprep ID:

SP002552

Sampleprep Summary:

Lipids were extracted from 100uL of brain homogenate using a two-phase methyl-tert-butyl ether (MTBE)/methanol/water protocol as described by Matyash et al. (2008), with the addition of the following internal standards: 2000 pmoles SM(d18:1_12:0), 2000 pmoles GluCer(d18:1_12:0), 500 pmoles LacCer(d18:1_12:0), 500 pmoles ST(18:1_17:0), 500 pmoles Cer(18:1/17:0), 200 pmoles C17:0 Sph, 200 pmoles C17:1 S1P, 200 pmoles d3-C16 AcCa, 500 pmoles C17:1 LPE, 500 pmoles C17:1 LPS, 200 pmoles C17:0 LPA, 500 pmoles C17:0 LPC, 5000 pmoles PC(19:0_19:0), 2000 pmoles PS(17:0_17:0), 2000 pmoles C17:0 PE, 2000 pmoles C17:0 PG, 1000 pmoles C17:0 PA, 1000 pmoles d7-18:1_15:0 PI, 2000 pmoles CL(14:0_14:0_14:0_14:0), 2000 pmoles TAG(17:0_17:0_17:0), 500 pmoles DAG d7- 18:1/15:0), 500 pmoles d7- 18:1 MAG, 2000 pmoles C17:0 CholE, 1000 pmoles d7 Chol. Lipids were reconstituted in 400uL of 100% HPLC methanol, then diluted 1/5 in 80% v/v HPLC methanol, containing 1 mM ammonium formate and 0.2% formic acid.