Summary of Study ST002508

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001619. The data can be accessed directly via it's Project DOI: 10.21228/M8F41P This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002508
Study Title	13C-isotopic labeling of mouse eye organoids under three conditions.
Study Summary	To examine the potential involvement of metabolites, we performed 13C-glucose labeling under three conditions: 13C-glucose = control, 13C-glucose + GNE-140 = LHDi, 13C-lactate in glucose free media mimicking the lack of glucose. Cell Name AES0145 : Rx-GFP K/I EB5 (RIKEN Cell Bank): Organoid method (PMID: 21475194).
Institute	Northwestern University
Last Name	TAKATA
First Name	NOZOMU
Address	303 East Superior Street, 10-220, Chicago, Illinois, 60611, USA
Email	nozomu.takata@northwestern.edu
Phone	13125036066
Submit Date	2023-03-15
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2023-06-01
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002614
Sampleprep Summary:	The eye organoids were collected and washed twice with PBS before pellets were flash-frozen and stored at −80°C until metabolite extraction. Metabolite analysis was performed as described previously (PMID: 33931446). Briefly, for metabolite extraction, 80% methanol was used followed by the rapid freeze-thaw method to break the tissues. The supernatant underwent speedvac drying. The samples were prepared in 80% acetonitrile and were analyzed by High-Resolution Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS).

Sampleprep ID:

SP002614

Sampleprep Summary:

The eye organoids were collected and washed twice with PBS before pellets were flash-frozen and stored at −80°C until metabolite extraction. Metabolite analysis was performed as described previously (PMID: 33931446). Briefly, for metabolite extraction, 80% methanol was used followed by the rapid freeze-thaw method to break the tissues. The supernatant underwent speedvac drying. The samples were prepared in 80% acetonitrile and were analyzed by High-Resolution Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS).