Summary of Study ST002528
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001627. The data can be accessed directly via it's Project DOI: 10.21228/M8D434 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002528 |
Study Title | Concentrations of amino acids in interstitial fluid and whole tumor samples of a murine PDAC orthotopic tumor model, measured by LC-MS |
Study Summary | We extracted polar metabolites from the interstitial fluid and whole tumor samples of orthotopic murine PDAC tumors. Weused LC-MS to measure the concentration of amino acids in the interstitial fluid of orthotopic murine PDAC tumors and compared this to the intratumoral concentrations. |
Institute | University of Chicago |
Last Name | Apiz Saab |
First Name | Juan |
Address | 929 E. 57th St. |
japizsaab@uchicago.edu | |
Phone | 7738346506 |
Submit Date | 2023-03-23 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2023-04-13 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP002634 |
Sampleprep Summary: | Cryogenically frozen tumor pieces were ground to a fine homogenous powder with a liquid nitrogen cooled mortar and pestle. ~30mg of tissue powder was weighed into sample tubes, and metabolites were extracted with 600µL HPLC grade methanol, 300µL HPLC grade water, and 400µL chloroform. Samples were vortexed for 10min at 4°C, centrifuged 21,000xg at 4°C for 10 min. 400µL of the aqueous top layer was removed into a new tube and dried under nitrogen. Dried tumor extracts were resuspended in 100µL HPLC grade water and LC-MS analysis was performed. For TIF samples, we extracted polar metabolites from 5µL of sample using 45µL of a 75:25:0.1 HPLC grade acetonitrile:methanol:formic acid extraction mix. Samples in extraction mix were vortexed for 10 min at 4°C and centrifugated at 15,000x rpm for 10 min at 4°C to pellet insoluble material. 20µL of the soluble polar metabolite supernatant was moved to sample vials for analysis by LC-MS. |