Summary of Study ST002533
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001629. The data can be accessed directly via it's Project DOI: 10.21228/M84M8C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002533 |
| Study Title | Untargeted Metabolomics on First Trimester Serum Implicates Metabolic Perturbations Associated with BMI in Development of Hypertensive Disorders: A Discovery Study |
| Study Summary | Body mass index (BMI) in early pregnancy is a critical risk factor for hypertensive disorders of pregnancy (HDP). The pathobiology of the interplay between BMI and HDP is not fully understood and represents the focus of this investigation. BMI and 1st-trimester serum samples were obtained from the Global Alliance to Prevent Prematurity and Stillbirth repository for 154 women (105 without HDP and 49 with HDP). Metabotyping was conducted using ultra-high-performance liquid-chromatography high-resolution mass spectrometry (UHPLC HR-MS). Regression models were used to determine metabolites and pathway perturbations associated with BMI in women with and without HDP, and to determine metabolites and pathway perturbations associated with HDP for women in categories of obese, overweight, and normal weight based on the 1st trimester BMI. This study will lay the groundwork for larger studies of predictive markers and biological pathways leading to infant morbidity and mortality. |
| Institute | University of North Carolina at Chapel Hill |
| Department | Nutrition |
| Last Name | Sumner |
| First Name | Susan |
| Address | 500 Laureate Way, Kannapolis, NC 28081 |
| susan_sumner@unc.edu | |
| Phone | 704-250-5066 |
| Submit Date | 2023-03-22 |
| Num Groups | 2 |
| Total Subjects | 154 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-07-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP002639 |
| Sampleprep Summary: | Samples were stored at -80 °C in the Global Alliance to Prevent Prematurity and Stillbirth (GAPPS) repository before being shipped (on dry ice) to Nutrition Research Institute, UNC-CH, for metabolomics analysis. Serum samples were thawed at 4 °C overnight. Samples were vortexed via a multiple-tube vortex mixer for 2 min at 5000 rpm and then sat on ice for 10 min before sample preparation. Quality control samples (QC pools) were made by pooling 7-µL serum from each parent sample (if sufficient volume is available). An aliquot of the sample (50-µL), including the study sample and QC pool, was transferred into a new pre-labeled tube and mixed with 400-µL methanol containing 500 ng/ml L-tryptophan-d5, vortexed at 5,000 rpm for 2 min, for protein precipitation. All samples were centrifuged at 16, 000 rcf for 10 min at 4°C. The supernatant (350-uL) was dried by a SpeedVac overnight. The dried extracts were reconstituted with 100 µl water-methanol (95:5, v/v) by thoroughly mixing on multiple tube vortex mixer for 10 min at 5000 rpm and centrifuging at 4°C for 10 min at 16,000 rcf. The supernatant (about 100 µL) was transferred to pre-labeled autosampler vials for data acquisition by LC-MS. |
| Processing Storage Conditions: | On ice |
| Extraction Method: | Vortex with methanol containing 500ng/ml tryptophan-d5 as internal standard |
| Extract Storage: | -80℃ |
| Sample Resuspension: | Water-Methanol (95:5, v/v) |
| Sample Spiking: | Tryptophan-d5 stock solution at 500 ng/mL |
