Summary of Study ST002547

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001640. The data can be accessed directly via it's Project DOI: 10.21228/M8QF0W This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002547
Study TitleC57bl/6 mice subjected to either PBS or bleomycin treatment to develop fibrosis
Study SummaryC57bl/6 mice subjected to either PBS or bleomycin treatment. By day 21, lung fibrosis will develop. We resected the lung and performed LC-QTOF analysis on small molecule metabolites between the two groups.
University of Florida
Last NameSun
First NameRamon
Address1200 Newell Drive, ARB, Gainesville, FL, 32610, USA
Submit Date2023-04-06
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2023-04-20
Release Version1
Ramon Sun Ramon Sun application/zip

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Sample Preparation:

Sampleprep ID:SP002653
Sampleprep Summary:Approximately 20mg of pulverized, frozen tissue was extracted in 3ml ice-cold 60% acetonitrile by extensively vortexing. Next, 1ml chloroform was added to each sample and samples were mixed by manual shaking followed by centrifuging at 3,200g for 20 minutes at 4°C. The aqueous layer was moved to a new tube and lyophilized. The middle layer containing protein and insoluble material was transferred to a new tube, washed twice with 50% methanol, once with 100% methanol, and briefly dried in a speed-vac. After drying, the insoluble material was hydrolyzed by heating samples in 3N HCl at 95°C for 2 hours. 100% methanol was added to hydrolysate to achieve a final concentration of 50% methanol, samples mixed, centrifuged at 18,000g for 10 min at 4°C, then supernatant dried on a speed vac.
Processing Storage Conditions:-80℃
Extract Storage:-80℃