Summary of Study ST002737
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001702. The data can be accessed directly via it's Project DOI: 10.21228/M8Q42J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002737 |
Study Title | 2’-fucosyllactose prevents colitis |
Study Type | untargeted metabolomics analysis |
Study Summary | Human milk-derived 2’-fucosyllactose (2’-FL) consumption is associated with health benefits in infancy that extend into adulthood. However, the exact biological functions of 2’-FL and corresponding mechanisms of action remain largely unknown. Here, we investigated the impact of 2’-FL on gut microbial metabolism for the prevention of colitis in adulthood. The gut microbiota from adult mice treated with 2’-FL showed an increase in abundance of several health-associated genera, including Bifidobacterium, and exhibited preventive effects on colitis. Microbial metabolic analysis demonstrated that 26 pathways that are significantly different between non-inflammatory bowel disease individuals and patients with ulcerative colitis (UC) are significantly regulated by 2’-FL in mice, indicating that 2’-FL has the potential to directly regulate dysregulated microbial metabolism in UC. Exploratory metabolomics of Bifidobacterium infantis identified novel secreted metabolites significantly enriched by 2’-FL consumption, including pantothenol. Remarkably, pantothenate significantly protects mucosal barrier and mitigates colitis in adult mice. Thus 2’-FL-modulated gut microbial metabolism may contribute to the prevention of intestinal inflammation in adulthood. |
Institute | Vanderbilt University |
Department | Chemistry |
Laboratory | Center for Innovative Technology |
Last Name | CODREANU |
First Name | SIMONA |
Address | 1234 STEVENSON CENTER LANE |
SIMONA.CODREANU@VANDERBILT.EDU | |
Phone | 6158758422 |
Submit Date | 2023-06-15 |
Num Groups | 2 |
Total Subjects | 10 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2023-12-18 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP002850 |
Sampleprep Summary: | Bifidobacteria infantis were cultured in Reinforced Clostridial Medium (RCM) with or without 2’-fucosyllactose (2’-FL), a human milk oligosaccharide. Bacteria were pelleted by centrifugation and supernatants were then collected, snap frozen, and stored at -80°C until analyzed via Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS and LC-HRMS/MS)-based metabolomics in the Vanderbilt Center for Innovative Technology using previously described methods. Briefly, equal volumes (200µL) of previously frozen culture medium were prepared. Isotopically labeled standards, biotin-D2 and phenylalanine-D8, were added to individual samples to assess the sample preparation steps. Samples were subjected to protein precipitation by addition of 800µL of ice-cold methanol, then incubated at -80C overnight. Following protein precipitation, samples were centrifuged at 10,000 rpm for 10 min to remove insoluble material. Supernatant(s) were transferred and dried in vacuo, and stored at -80C prior to MS characterization. |
Processing Storage Conditions: | -80℃ |
Extraction Method: | Following lysis and standard addition, protein precipitation was performed by adding 800µL of ice-cold methanol (4x by volume). Samples were incubated at -80°C overnight. Following incubation, samples were centrifuged at 10,000 rpm for 10 min to eliminate proteins. The supernatants containing metabolites were dried via speed-vacuum. |
Extract Storage: | -80℃ |