Summary of Study ST002754

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001715. The data can be accessed directly via it's Project DOI: 10.21228/M81D9R This work is supported by NIH grant, U2C- DK119886.


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Study IDST002754
Study TitleMetabolomics analysis of maternal obesity model
Study Summary100μl serum of 11-12 weeks old male mice with a background from various diet groups were processed in Metabolon ( for metabolics analysis.Diet groups are CDm CDl CD, CDm CDl HFD, CDmHFDl HFD, HFDm CDl CD, HFDm CDl HFD, HFDm HFDl CD and HFDm CDl CD. (m: maternal diet, l: lactation)
University of Bonn
LaboratoryMass Lab
Last NameMass
First NameElvira
AddressLIMES-Institute, Carl-Troll-Str. 31, 53115 Bonn, Germany
Phone+49 02 28 / 73 6 28 48
Submit Date2023-06-14
Num Groups6
Total Subjects42
Num Males42
Analysis Type DetailLC-MS
Release Date2023-08-01
Release Version1
Elvira Mass Elvira Mass application/zip

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Sample Preparation:

Sampleprep ID:SP002867
Sampleprep Summary:Sera were taken and sent to Metabolon. Further sample preparation was done on Metabolon Site. In short, samples were prepared using the automated MicroLab STAR® system from Hamilton Company. Several recovery standards were added prior to the first step in the extraction process for QC purposes. To remove protein, dissociate small molecules bound to protein or trapped in the precipitated protein matrix, and to recover chemically diverse metabolites, proteins were precipitated with methanol under vigorous shaking for 2 min (Glen Mills GenoGrinder 2000) followed by centrifugation. The resulting extract was divided into five fractions: two for analysis by two separate reverse phase (RP)/UPLC-MS/MS methods with positive ion mode electrospray ionization (ESI), one for analysis by RP/UPLC-MS/MS with negative ion mode ESI, one for analysis by HILIC/UPLC-MS/MS with negative ion mode ESI, and one sample was reserved for backup. Samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. The sample extracts were stored overnight under nitrogen before preparation for analysis.