Summary of Study ST002754

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001715. The data can be accessed directly via it's Project DOI: 10.21228/M81D9R This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST002754
Study Title	Metabolomics analysis of maternal obesity model
Study Summary	100μl serum of 11-12 weeks old male mice with a background from various diet groups were processed in Metabolon (https://www.metabolon.com) for metabolics analysis.Diet groups are CDm CDl CD, CDm CDl HFD, CDmHFDl HFD, HFDm CDl CD, HFDm CDl HFD, HFDm HFDl CD and HFDm CDl CD. (m: maternal diet, l: lactation)
Institute	University of Bonn
Department	LIMES
Laboratory	Mass Lab
Last Name	Mass
First Name	Elvira
Address	LIMES-Institute, Carl-Troll-Str. 31, 53115 Bonn, Germany
Email	elvira.mass@uni-bonn.de
Phone	+49 02 28 / 73 6 28 48
Submit Date	2023-06-14
Num Groups	6
Total Subjects	42
Num Males	42
Analysis Type Detail	LC-MS
Release Date	2023-08-01
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002867
Sampleprep Summary:	Sera were taken and sent to Metabolon. Further sample preparation was done on Metabolon Site. In short, samples were prepared using the automated MicroLab STAR® system from Hamilton Company. Several recovery standards were added prior to the first step in the extraction process for QC purposes. To remove protein, dissociate small molecules bound to protein or trapped in the precipitated protein matrix, and to recover chemically diverse metabolites, proteins were precipitated with methanol under vigorous shaking for 2 min (Glen Mills GenoGrinder 2000) followed by centrifugation. The resulting extract was divided into five fractions: two for analysis by two separate reverse phase (RP)/UPLC-MS/MS methods with positive ion mode electrospray ionization (ESI), one for analysis by RP/UPLC-MS/MS with negative ion mode ESI, one for analysis by HILIC/UPLC-MS/MS with negative ion mode ESI, and one sample was reserved for backup. Samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. The sample extracts were stored overnight under nitrogen before preparation for analysis.

Sampleprep ID:

SP002867

Sampleprep Summary:

Sera were taken and sent to Metabolon. Further sample preparation was done on Metabolon Site. In short, samples were prepared using the automated MicroLab STAR® system from Hamilton Company. Several recovery standards were added prior to the first step in the extraction process for QC purposes. To remove protein, dissociate small molecules bound to protein or trapped in the precipitated protein matrix, and to recover chemically diverse metabolites, proteins were precipitated with methanol under vigorous shaking for 2 min (Glen Mills GenoGrinder 2000) followed by centrifugation. The resulting extract was divided into five fractions: two for analysis by two separate reverse phase (RP)/UPLC-MS/MS methods with positive ion mode electrospray ionization (ESI), one for analysis by RP/UPLC-MS/MS with negative ion mode ESI, one for analysis by HILIC/UPLC-MS/MS with negative ion mode ESI, and one sample was reserved for backup. Samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. The sample extracts were stored overnight under nitrogen before preparation for analysis.