Summary of Study ST002839
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001778. The data can be accessed directly via it's Project DOI: 10.21228/M8WB1S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002839 |
| Study Title | Metabolic alteration during ferroptotic process in cancer cells. |
| Study Summary | Targeting ferroptosis, an iron-dependent form of regulated cell death triggered by the lethal overload of lipid peroxides, in cancer therapy is impeded by our limited understanding of the intersection of tumour’s metabolic feature and ferroptosis vulnerability. In this study, we performed metabolomics in cancer cell lines pretreated with ferroptosis inducer RSL3 and control DMSO. We noted that the levels of a series of metabolites were significantly impacted by the RSL3 treatment, such as upregulated polyamines including putrescine, spermidine, and spermine. According to our previous study, we proved the pro-ferroptotic feature of polyamines in tumor cells, which was derived from the H2O2 produced during the polyamine metabolic process. This finding is consistent with our RNA-Seq data indicating upregulated ODC1 in the ferroptotic process. Therefore, it could be speculated that the RSL3-induced polyamine production leads to a positive-feedback loop that amplifies ferroptosis in tumor cells. |
| Institute | Zhongshan Hospital Fudan University |
| Last Name | Bi |
| First Name | Guoshu |
| Address | Zhongshan Hospital, Fudan University, No. 180, Fenglin Road, Xuhui District, Shanghai, China |
| 18211210035@fudan.edu.cn | |
| Phone | 86 64041990 |
| Submit Date | 2023-08-25 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-09-07 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP002955 |
| Sampleprep Summary: | After homogenization in liquid N2, 200 μL 80% methyl alcohol was added, followed by vortex for 1 min, sonication for 30 min at 4°C, and stand for 1 h at -20°C. Then, the samples were centrifuged at 12,000 rpm for 15 min at 4°C, and the supernatant was collected for subsequent LC-MS analysis. |
