Summary of Study ST002840
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001779. The data can be accessed directly via it's Project DOI: 10.21228/M8RM7K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002840 |
| Study Title | Collaborative role of YqgC and superoxide dismutase (MnSOD) in manganese intoxication: Replicate Experiment 1 |
| Study Type | Targeted MS Analysis |
| Study Summary | In this study, we are validating the mutual impact of yqgC and superoxide dismutase (MnSOD) deletion (YS strain) and resultant hypersensitivity to Manganese (Mn). Our genetic and physiological studies show that Mn intoxication is a result of distinct enzymatic vulnerabilities via alleviated expression of Mg-dependent, chorismate-utilizing enzymes of the menaquinone, siderophore, and tryptophan (MST) family (due to mismetalation). |
| Institute | Cornell University |
| Department | Medicine |
| Laboratory | Rhee Lab |
| Last Name | Soni |
| First Name | Vijay |
| Address | Weill Cornell Medical College, 413 E 69th St |
| vis2032@med.cornell.edu | |
| Phone | 9175391105 |
| Submit Date | 2023-08-28 |
| Num Groups | 10 |
| Total Subjects | 60 |
| Study Comments | NA |
| Publications | NA |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Chear Study | NA |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-09-21 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP002956 |
| Sampleprep Summary: | Samples were lysed using 0.1 mm Zirconia beads in a Precellys homogenizer. Lysed suspensions were centrifuged at 12,000 rpm for 8 min at 37 ˚C. Supernatants were passed through 0.22 um SpinX tube filter and flowthrough was collected for the LC-MS run. |
| Sampleprep Protocol ID: | NA |
| Sampleprep Protocol Filename: | NA |
| Sampleprep Protocol Comments: | NA |
| Processing Method: | NA |
| Processing Storage Conditions: | On ice |
| Extraction Method: | Beat beating |
| Extract Enrichment: | NA |
| Extract Cleanup: | NA |
| Extract Storage: | 4℃ |
| Sample Resuspension: | NA |
| Sample Derivatization: | NA |
| Sample Spiking: | NA |
| Subcellular Location: | NA |
