Summary of Study ST002859

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001788. The data can be accessed directly via it's Project DOI: 10.21228/M8KX5C This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002859
Study TitleGlucose tracing in vehicle- and Enzalutamide-treated 16D prostate cancer cells
Study SummaryIn this study, we sought to determine how Enzalutamide treatment alters metabolism in 16D prostate cancer cells.
Institute
University of California, Los Angeles
LaboratoryGoldstein Lab
Last NameGiafaglione
First NameJenna
Address610 Charles E Young Dr S, Los Angeles, CA, 90024, USA
Emailjgiafaglione@g.ucla.edu
Phone408-728-3065
Submit Date2023-09-12
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailGC-MS
Release Date2023-09-15
Release Version1
Jenna Giafaglione Jenna Giafaglione
https://dx.doi.org/10.21228/M8KX5C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP002977
Sampleprep Summary:Cell plates were placed on ice and quickly washed with ice-cold 0.9% (w/v) NaCl. Cells were immediately treated with 500 μL of ice-cold MeOH and 200 μL water containing 1 μg of the internal standard norvaline. Cells were then scraped and placed in 1.5 mL centrifuge tubes kept on ice. Next, 500 μL of chloroform was added, after which samples were vortexed for 1 minute and then spun at 10,000g for 5 minutes at 4C. The aqueous layer was transferred to a GC/MS sample vial and dried overnight using a refrigerated CentriVap.
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