Summary of Study ST002891
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001804. The data can be accessed directly via it's Project DOI: 10.21228/M8HX5R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002891 |
| Study Title | Glutamine metabolism improves left ventricular function but not macrophage-mediated inflammation following myocardial infarction |
| Study Type | untargeted metabolomics analysis |
| Study Summary | Glutamine is a critical amino acid that serves as an energy source, building block, and signaling molecule for both the heart tissue and the immune system. However, the role of glutamine metabolism in regulating cardiac remodeling following myocardial infarction (MI) is unknown. In this study, we show that glutamine metabolism is altered both in the remote (contractile) area and in infiltrating macrophages in the infarct area after MI in adult male mice by permanent left anterior descending artery occlusion. Using untargeted metabolomics in extracted LV macrophages, we found that metabolites related to glutamine metabolism were differentially altered at days 1, 3, and 7 after MI. Glutamine metabolism in live cells was found to be increased after MI relative to no MI controls. Gene expression in the remote area of the heart indicated a loss of glutamine metabolism. Glutamine administration improved LV function at days 1, 3, and 7, which was associated with improved contractile and metabolic gene expression. |
| Institute | Vanderbilt University |
| Department | Chemistry |
| Laboratory | Center for Innovative Technology |
| Last Name | Codreanu |
| First Name | Simona |
| Address | 1234 STEVENSON CENTER LANE, NASHVILLE, TN, 37235, USA |
| SIMONA.CODREANU@VANDERBILT.EDU | |
| Phone | 6158758422 |
| Submit Date | 2023-09-29 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-04-02 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP003010 |
| Sampleprep Summary: | Frozen samples were stored at -80°C until analyzed by LC-MS-based metabolomics in the Vanderbilt Center for Innovative Technology (CIT). Isotopically labeled phenylalanine-D8 and biotin-D2 were added to 200 μL of culture supernatant per sample, and protein was precipitated by addition of 800 μL of ice-cold methanol followed by overnight incubation at −80°C. Precipitated proteins were pelleted by centrifugation (15,000 rpm, 15 min), and extracted metabolites were dried down in vacuo and stored at −80°C. Individual samples were reconstituted in 100 μL of reconstitution buffer (acetonitrile/water, 90:10, vol/vol) containing tryptophan-D3 and inosine-4N15. Equal volumes of individual samples were pooled to create a quality control (QC) pooled sample used for column conditioning, retention time alignment, to assess instrument reproducibility, and for batch acceptance. |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -80℃ |
