Summary of Study ST002942
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001830. The data can be accessed directly via it's Project DOI: 10.21228/M85H91 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST002942 |
Study Title | Targeted analysis of short chain fatty acids for the XMaS clinical trial. |
Study Summary | The Xanthohumol microbiome and signature (XMaS) study in healthy adults was a phase I, triple-masked, placebo-controlled clinical trial in healthy adults investigating effects of a natural product supplement of gut microbiome composition and metabolism. Xanthohumol, a flavonoid from the hops plant or placebo was administered to 27 healthy adults daily for eight weeks. Fecal, plasma, and 24-hr urine collections were obtained from participants at baseline and 2-week intervals. The main objective of this study was to quantify short chain fatty acids from fecal samples of particiapnts in the XMaS trail |
Institute | Oregon State University |
Last Name | Jamieson |
First Name | Paige |
Address | 307 LINUS PAULING SCIENCE CTR |
jamiesop@oregonstate.edu | |
Phone | 5417379804 |
Submit Date | 2023-10-22 |
Analysis Type Detail | LC-MS |
Release Date | 2024-01-01 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP003061 |
Sampleprep Summary: | Fecal samples were dried using vacuum desiccator and 70% methanol:water (1:4 w/v) was added for fecal homogenate. Samples were centrifuged and supernatant was collected. Supernatant was diluted 1:5 in 50% acetonitrile:water and short chain fatty acids were derivatized with 3-nitrophenylhydrazine for better resolution by LC-MS. Briefly, 40 uL of fecal supernatant was added to 20 uL 120 mM EDC, 20 uL 6% pyridine, and 20 uL 200 mM 3-NPH. The mixture was allowed to react for 30 min at 40 °C and subsequently diluted 1:200 in 10% acetonitrile:water. |