Summary of Study ST002960
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001842. The data can be accessed directly via it's Project DOI: 10.21228/M8MH8P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002960 |
| Study Title | Analysis of Lipids Secreted from Fibroblast Young Cells |
| Study Summary | In this experimental study, we aimed to understand the potential factors within the secretions of young cells that could trigger the reverse aging of Mid-old cells. To investigate this phenomenon, we co-cultured young cells with Mid-old cells and observed a fascinating outcome: the Mid-old cells exhibited reverse aging and transformed into a more youthful state. To uncover the specific factors responsible for this reverse aging effect, we conducted a detailed analysis of the secreted factors from the young cells. Our analysis focused on a range of biomolecules, including lipids. However, despite our efforts, we did not identify any distinct factors that could be directly attributed to this remarkable reverse aging process. |
| Institute | Ajou University Medical Center |
| Last Name | Kim |
| First Name | Young Hwa |
| Address | 206, World cup-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do, Republic of Korea |
| skyblue32@nate.com | |
| Phone | +82-10-5153-3636 |
| Submit Date | 2023-11-01 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-11-22 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP003079 |
| Sampleprep Summary: | For lipid extraction from samples, a two-step method involving neutral and acidic extraction were used. At first, in neutral extraction, lipids from the samples were extracted according to the Folch method using a mixture of chloroform and methanol (2:1, v/v). The samples were vortexed and incubated on ice for 10 min. The samples were centrifuged at 13,800×g for 2 min at 4°C, supernatant was transferred to a new 1.5 mL tube. Next, in Acidic extraction, 750 μL of chloroform:methanol:HCl (1N, 37%) (40:80:1, v/v/v) was added to the remaining samples. After incubating for 15 min at room temperature, 250 μL of cold chloroform and 450 μL of cold 0.1 M HCl were added to the sample. The mixture was vortexed for 1 min and centrifuged at 6,500×g for 2 min at 4°C. The lower organic phase was collected and combined with the prior extract. The sample was then dried using HyperVAC-MAX VC2200 centrifugal vacuum concentrator (Hanil Scientific Inc., Korea). Dried metabolite contents were reconstituted in 50 µL of mobile phase solvent A:solvent B (2 :1, v/v) and then subjected to LC-MS/MS analysis. |
| Sampleprep Protocol Filename: | Sample_prep_lipid.pdf |
