Summary of Study ST002963

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001843. The data can be accessed directly via it's Project DOI: 10.21228/M8GT6S This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002963
Study Title	LC/MS detection for GSH and GSSG levels in KRAS-driven lung tumors with LKB1 or p53 deficiency, comparing cases with G6PD wild-type and G6PD knockout
Study Summary	In this study, LC/MS was employed to assess whether G6PD loss affects the GSH and GSSG pool in Lkb1-deficient KRAS-driven lung tumors with LKB1 or p53 deficiency.
Institute	Rutgers Cancer Institute of New Jersey
Last Name	Guo
First Name	Jessie
Address	Room 3020, 195 Little Albany Stree (Rutgers Cancer Institute of New Jersey)
Email	yanxiang@cinj.rutgers.edu
Phone	17327632262
Submit Date	2023-10-26
Raw Data Available	Yes
Raw Data File Type(s)	mzXML
Analysis Type Detail	LC-MS
Release Date	2024-01-16
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP003082
Sampleprep Summary:	Approximately 20 mg of tumor samples were precisely weighed and placed into a pre-cooled tube. The tissue samples were then pulverized using the Cryomill. Pre-cooled extraction buffer consisting of methanol: acetonitrile: H2O (40:40:20, V/V) with 0.5% formic acid (Sigma-Aldrich, # F0507-100ML) was added to the resulting powder (40 μL of solvent per mg of tumors). The samples were then vortexed for 15 seconds and incubated on ice for 10 minutes. Subsequently, 15% NH4HCO3 solution (5% V/V of the extraction buffer) was used to neutralize the samples. Then all samples were vortexed again for 10 seconds and centrifuged at 4°C, 13,000 × g for 20 minutes. The resulting supernatant was transferred to LC-MS vials for subsequent analysis.

Sampleprep ID:

SP003082

Sampleprep Summary:

Approximately 20 mg of tumor samples were precisely weighed and placed into a pre-cooled tube. The tissue samples were then pulverized using the Cryomill. Pre-cooled extraction buffer consisting of methanol: acetonitrile: H2O (40:40:20, V/V) with 0.5% formic acid (Sigma-Aldrich, # F0507-100ML) was added to the resulting powder (40 μL of solvent per mg of tumors). The samples were then vortexed for 15 seconds and incubated on ice for 10 minutes. Subsequently, 15% NH4HCO3 solution (5% V/V of the extraction buffer) was used to neutralize the samples. Then all samples were vortexed again for 10 seconds and centrifuged at 4°C, 13,000 × g for 20 minutes. The resulting supernatant was transferred to LC-MS vials for subsequent analysis.